Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study  

作　　者：C.Almiñana F.Dubuisson S.Bauersachs E.Royer P.Mermillod E.Blesbois F.Guignot 

机构地区：[1]UMR PRC,INRAE 0085,CNRS 7247,Universitéde Tours,IFCE,F,–37380 Nouzilly,France [2]Functional Genomics Group,Institute of Veterinary Anatomy,VetSuisse Faculty Zurich,University of Zurich,Zürich,Switzerland [3]UEPAO,INRAE,F,–37380 Nouzilly,France

出　　处：《Journal of Animal Science and Biotechnology》2022年第3期735-755,共21页畜牧与生物技术杂志（英文版）

基　　金：funded by EU in the framework of H2020–SFS–2015–2under grant agreement IMAGE–677353–2;supported by COST–Action SLAAM–COST–STSM–BM1308–36887。

摘　　要：Background:Currently,there is a high demand for efficient pig embryo cryopreservation procedures in the porcine industry as well as for genetic diversity preservation and research purposes.To date,vitrification(VIT)is the most efficient method for pig embryo cryopreservation.Despite a high number of embryos survives in vitro after vitrification/warming procedures,the in vivo embryo survival rates after embryo transfer are variable among laboratories.So far,most studies have focused on cryoprotective agents and devices,while the VIT effects on porcine embryonic gene expression remained unclear.The few studies performed were based on vitrified/warmed embryos that were cultured in vitro(IVC)to allow them to re–expand.Thus,the specific alterations of VIT,IVC,and the cumulative effect of both remained unknown.To unveil the VIT-specific embryonic alterations,gene expression in VIT versus(vs.)IVC embryos was analyzed.Additionally,changes derived from both VIT and IVC vs.control embryos(CO)were analyzed to confirm the VIT embryonic alterations.Three groups of in vivo embryos at the blastocyst stage were analyzed by RNA–sequencing:(1)VIT embryos(vitrified/warmed and cultured in vitro),(2)IVC embryos and(3)CO embryos.Results:RNA–sequencing revealed three clearly different mRNA profiles for VIT,IVC and CO embryos.Comparative analysis of mRNA profiles between VIT and IVC identified 321,differentially expressed genes(DEG)(FDR<0.006).In VIT vs.CO and IVC vs.CO,1901 and 1519 DEG were found,respectively,with an overlap of 1045 genes.VIT-specific functional alterations were associated to response to osmotic stress,response to hormones,and developmental growth.While alterations in response to hypoxia and mitophagy were related to the sum of VIT and IVC effects.Conclusions:Our findings revealed new insights into the VIT procedure-specific alterations of embryonic gene expression by first comparing differences in VIT vs.IVC embryos and second by an integrative transcriptome analysis including in vivo control embryos.The identi

关 键 词：BLASTOCYST Embryo Gene expression In vitro culture PORCINE RNA–sequencing TRANSCRIPTOMICS VITRIFICATION 

分 类 号：S828[农业科学—畜牧学]