男性血清降钙素基因相关肽与体外受精胚胎培养结局的相关性研究  

Study on the correlation between male serum calcitonin gene-related peptide and the embryo culture outcome after in vitro fertilization

作　　者：陈佳静 陈剑锋[1] 陈佳丽 陈淑贤[1] 刘智任 尤沁媛 张翠岚 刘奇才[1] Chen Jiajing;Chen Jianfeng;Chen Jiali;Chen Shuxian;Liu Zhiren;You Qinyuan;Zhang Cuilan;Liu Qicai(Center for Reproductive Medicine,1st Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China;Department of Electrocardiograph,Fujian Hospital,Huashan Hospital affiliated to Fudan University,Fuzhou 351105,China)

机构地区：[1]福建医科大学附属第一医院生殖医学中心,福州350005 [2]复旦大学附属华山医院福建医院心电图室,福州351105

出　　处：《中华检验医学杂志》2022年第5期439-443,共5页Chinese Journal of Laboratory Medicine

基　　金：福建省自然科学基金(2021J02036,2021J01242);吴阶平医学基金会临床科研专项(320.6750.2021-19-4)。

摘　　要：目的分析不育症男性患者血清降钙素基因相关肽(CGRP)水平和体外受精胚胎培养结局的相关性。方法对2019年7至10月于福建医科大学附属第一医院生殖医学中心就诊患者中随机抽样25例接受体外受精胚胎移植辅助生殖治疗的男性患者进行研究,以血清CGRP对数转化指标、受精方式、是否取精困难、年龄、不育年限、泌乳素等为自变量,以总受精率、正常受精率、D3优质胚胎率、囊胚形成率为因变量,运用Pearson相关分析和多元回归法,进行相关性分析。结果Pearson相关分析显示,原发不育组中D3优质胚胎率与精子正常形态率相关(r=0.537,P=0.048),囊胚形成率与精子密度相关(r=0.760,P=0.002),CGRP对数与总受精率相关(r=0.693,P=0.006)。在继发不育组中,总受精率及正常受精率与精子总数相关(r=0.614,P=0.042;r=0.611,P=0.046)。多元回归分析显示,在继发不育组中,正常受精率与精子总数、血清CGRP对数转换指标、精子正常形态率之间有线性关系,且精子总数、CGRP对数转化指标与精子正常形态率的标准化回归系数分别为0.2、-72.9、6.8。结论男性的血清CGRP、精子总数和精子正常形态率共同影响正常受精率。Objective To investigate the correlation between serum calcitonin gene-related peptide(CGRP)and the culture outcome of in vitro fertilization embryo in male patients with infertility.Methods In this study,the randomized samples from 25 male patients who received in vitro fertilization-embryo transfer(IVF-ET)in the 1st Affiliated Hospital of Fujian Medical University from June 2019 to October 2019 were analyzed by multiple linear stepwise regression,with some important clinical outcomes,such as the logarithmic conversion index of serum CGRP,fertilization method,masturbation difficulty,age,infertility duration,and prolactin,as independent variables,while total fertilization rate,normal fertilization rate,high quality embryo rate at day 3,blastocyst formation rate as dependent variables.Results The Pearson correlation analysis showed that the D3 high-quality embryo rate was related to the normal sperm morphology rate in the primary infertility group(r=0.537,P=0.048),the blastocyst formation rate was correlated with sperm density(r=0.760,P=0.002),the CGRP logarithm was correlated with the total fertilization rate(r=0.693,P=0.006).The logarithmic conversion index of serum CGRP was related to the total fertilization rate and normal fertilization rate in the secondary infertility group(r=0.614,P=0.042 and r=0.611,P=0.046).In the secondary infertility group,there was a linear relationship between normal fertilization rate and total sperm count,serum CGRP log conversion,and sperm normal morphology rate,with standardized regression coefficients of 0.2,-0.729,and 6.8,respectively.Conclusion Serum CGRP level,together with total sperm count and normal sperm morphology rate may affects normal fertilization rate in male patients with infertility.

关 键 词：降钙素基因相关肽 不育 男(雄)性 体外受精 正常受精率 

分 类 号：R714.8[医药卫生—妇产科学]