川椒抑制木材腐朽菌性能研究  

Optimization of Extraction Process of Zanthoxylum by Response Surface Methodology and Its Antibacterial Performance

作　　者：盛婧源 王长健 王雅梅[1] 任士明 赵晓琪 SHENG Jingyuan;WANG Changjian;WANG Yamei;REN Shiming;ZHAO Xiaoqi(College of Materials Science and Art Design,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学材料科学与艺术设计学院,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2022年第2期107-114,共8页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：内蒙古自治区科技创新引导项目;内蒙古自治区自然科学基金项目(2018MS03048)。

摘　　要：本试验采用超声辅助回流法提取川椒有效抑菌成分,以彩绒革盖菌和密粘褶菌的菌丝生长抑制率为指标进行提取工艺的优化。采用了响应面分析法确定川椒提取的最佳工艺条件,同时探讨了液料比、乙醇浓度、提取时间和温度间的显著性影响和交互作用。当乙醇浓度95%,液料比12∶1,超声时间40.00 min,超声温度45.0℃,对彩绒革盖菌生长抑制率为26.35%;当乙醇浓度95%,液料比20∶1,超声时间53.00 min,超声温度65.0℃,对密粘褶菌生长抑制率为66.13%;当乙醇浓度95%,液料比20∶1,超声时间59.00 min,超声温度65.0℃,彩绒革盖菌生长抑制率为21.39%,密粘褶菌生长抑制率为63.54%。In this experiment,the effective antibacterial components of Zanthoxylum were extracted by the ultrasound-assisted reflux method,and the extraction process was optimized by takine the inhibition rate of hyphae growth of Trametes versicolor and Gloeophyllum trabeum as the index.The optimum extraction conditions of Zanthoxylum were determined by the response surface analysis method,and the significant effects and interactions of the liquid-solid ratio,ethanol concentration,extraction time and temperature were also discussed.When the ethanol concentration was 95%,the liquid-material ratio was 12∶1,the ultrasonic time was 40.00 min,and the ultrasonic temperature was 45.0℃,the growth inhibition rate of Trametes versicolor was 26.35%.When the ethanol concentration was 95%,the liquid-material ratio was 20∶1,the ultrasonic time was 53.00 min,and the ultrasonic temperature was 65.0℃,the growth inhibition rate of Gloeophyllum trabeum was 66.13%.When the ethanol concentration was 95%,the liquid-material ratio was20∶1,the ultrasonic time was 59.00 min,and the ultrasonic temperature was 65.0℃,the growth inhibition rate of trichoplegia was21.39%,and the growth inhibition rate of Trametes versicolor was 63.54%.

关 键 词：川椒 响应面法 彩绒革盖菌 密粘褶菌 菌丝生长速率法 

分 类 号：S781.72[农业科学—木材科学与技术]