不同产地五指毛桃根中补骨脂素、芹菜素、佛手柑内酯含量的比较  

Comparison of contents of psoralen,apigenin and bergapten in the root of ficus hirta vahl from different origins

作　　者：陈荣珠[1] 王小平[1] 高伟城[1] 李珍[1] CHEN Rongzhu

机构地区：[1]漳州卫生职业学院,福建漳州363000

出　　处：《中医临床研究》2022年第8期124-127,共4页Clinical Journal Of Chinese Medicine

基　　金：2019年医疗服务与保障能力提升补助资金“全国中药资源普查项目”(财社[2019]39号);漳州市自然科学基金项目(ZZ2021J16);漳州卫生职业学院高学历人才科研支持专项(ZWYGXL202101)。

摘　　要：目的:用高效液相色谱法同时测定不同产地五指毛桃(Ficus hirta Vahl)根中补骨脂素、芹菜素和佛手柑内酯的含量。方法:采用高效液相色谱法,色谱柱为HD-C18-80(4.6 mm×250 mm,5μm);检测波长为254 nm,流动相为甲醇-水(55∶45);流速1.0 mL/min。结果:不同产地五指毛桃根中补骨脂素、芹菜素和佛手柑内酯的含量差异较大,其中以广西钦州市灵山县五指毛桃根中含量最高,而福建省漳州市龙文区五指毛桃根中的含量最低。结论:该方法操作简便,重复性好,可用于五指毛桃根中补骨脂素、芹菜素和佛手柑内酯含量的测定。Objective:The high performance liquid chromatography method was used to simultaneously determine the contents of psoralen,apigenin and bergapten in the root of ficus hirta vahl from different origins.Methods:HPLC method was performed on the column of HD-C18-80(4.6 mm×250 mm,5μm).The detection wavelength was 254 nm,and the mobile phase was methanol-water(55:45).The flow rate was 1.0 mL/min.Results:The contents of psoralen,apigenin and bergapten in the roots of ficus hirta vahl from different origins were significantly different.Among them,the three compounds in the roots of ficus hirta vahl from Lingshan county,Qinzhou city,Guangxi Province were the highest,and those from Longwen district,Zhangzhou city,Fujian Province were the lowest.Conclusion:The method is easy to operate and has good repeatability,so can be used for the content determination of psoralen,apigenin and bergapten in the root of ficus hirta vahl.

关 键 词：五指毛桃 高效液相色谱法 补骨脂素 芹菜素 佛手柑内酯 

分 类 号：R284.1[医药卫生—中药学]