甘蔗类质子梯度调节蛋白基因的生物信息学及表达分析  

The Bioinformatics and Expression Analysis of Sugarcane Proton Gradient Regulation Like 1 Gene

作　　者：黄宁 范金燕 赖洁玲[1] 刘秦 阙友雄[2] 朱宇林[1] 凌辉 Huang Ning;Fan Jinyan;Lai Jieling;Liu Qin;Que Youxiong;Zhu Yulin;Ling Hui(College of Agriculture,Yulin Normal University,Yulin,537000;National Engineering Research Center for Sugarcane,Fujian Agriculture and Forestry University,Fuzhou,350002)

机构地区：[1]玉林师范学院农学院,玉林537000 [2]福建农林大学,国家甘蔗工程技术研究中心,福州350002

出　　处：《分子植物育种》2022年第9期2876-2884,共9页Molecular Plant Breeding

基　　金：玉林师范学院高层次人才启动项目(G2020ZK03);国家自然科学基金青年科学基金项目(31901592);经济作物遗传改良与综合利用湖南省重点实验室开放基金项目(E22009)共同资助。

摘　　要：PGR5(Proton gradient regulation 5)/PGRL1(Proton gradient regulation like 1)介导的环式电子传递(cyclic electron transfer,CET)在高等植物调节光合作用、缓解光抑制过程中起着重要作用。目前,在甘蔗(Saccharum spp.)中还未见关于PGRL1的研究。本研究克隆得到一个甘蔗ScPGRL1基因(GenBank accession number:MT747432),生物信息分析结果显示,ScPGRL1包含一个983 bp的完整开放阅读框,编码327个氨基酸的不稳定酸性蛋白。ScPGRL1蛋白主要由无规则卷曲、α-螺旋和延伸链组成,具有ATP合酶活性位点、肽配体结合位点和两个跨膜区。系统进化树分析表明,甘蔗ScPGRL1与高粱和玉米PGRL1在系统发育分支中距离最近,且“GPRCS”氨基酸序列及PGRL1蛋白的两个跨膜区在植物进化过程中保守。亚细胞定位结果表明,ScPGRL1定位于细胞膜和叶绿体。实时荧光定量PCR分析表明,ScPGRL1基因在茉莉酸甲酯胁迫下被显著诱导表达,在双氧水胁迫下被显著抑制表达。本研究为深入研究ScPGRL1基因的功能和作用模式奠定了基础。The cyclic electron transfer(CET)mediated by PGR5(Proton gradient re gulation 5)/PGRL1(Proton gradient regulation like 1)plays a major role in photosynthesis regulation and photo inhibition remission in higher plants.At present,there is no report referring to PGRL1 in sugarcane(Saccharum spp.).A sugarcane ScPGRL1 gene was cloned(GenBank accession number:MT747432)and sequenced in the present study.Sequence analysis suggested that the cDNA sequence of the ScPGRL1 gene contained a complete open reading frame of 983 bp,which encoded an unstable acidic protein with 327 amino acid residues.ScPGRL1 contained random coil,alpha helix,and extension chains in its secondary structure,while its three-dimensional structure was presumed to consist of an ATP synthase active site,a peptide ligand binding site and two transmembrane domains.Phylogenetic tree showed that ScPGRL1 was closest to PGRL1 from sorghum and maize in phylogenetic relationship,and its"GPRCS"motif and transmembrane domains were the highly conserved regions in evolution.The transient expression of the ScPGRL1 gene in Nicotiana benthamiana leaves indicated that ScPGRL1 protein was located in the cell membrane and chloroplast.Real-time quantitative PCR analysis showed that the expression of ScPGRL1was significantly up-regulated by Me JA treatment,and down-regulated by H;O;stress.This study lays a foundation for further study on the function and regulation pattern of ScPGRL1 gene.

关 键 词：甘蔗 PGRL1 生物信息学 光抑制 亚细胞定位 

分 类 号：S566.1[农业科学—作物学]