丹参BZR基因家族的全基因组鉴定及生物信息学分析  

Genome-wide Iden tification and Bioinformatics Analysis of SmBZR Gene Family in Salvia miltiorrhiza

作　　者：杜博轩 张海花[1] 杨东风[1] 梁宗锁[1] 金伟波[1] Du Boxuan;Zhang Haihua;Yang Dongfeng;Liang Zongsuo;Jin Weibo(Key Laboratory of Plant Secondary Metabolism and Regulation of Zheyiang Province,College of Life Sciences and Medicine,Zhejiang Sci-Tech Uni­versity,Hangzhou,310018)

机构地区：[1]浙江理工大学生命科学与医药学院浙江省植物次生代谢调控重点实验室,杭州310018

出　　处：《分子植物育种》2022年第9期2891-2898,共8页Molecular Plant Breeding

基　　金：浙江省自然科学基金项目(LY18C150010);浙江理工大学521人才基金项目共同资助。

摘　　要：油菜素内酯(brassinosteroid,BR)是一类植物类固醇激素,除了具有生长调节作用外,还具有抗逆功能。BZR(BRASSINAZOLE-RESISTANT)转录因子是油菜素内酯信号转导途径中的调节剂,对植物生长和抵抗环境刺激起着至关重要的作用。本研究对丹参(Salvia miltiorrhiza Bunge)BZR成员的全基因组进行鉴定,鉴定得到6个成员。通过一系列生物信息学分析对它们的基因结构、进化关系、保守基序、顺式作用元件和茉莉酸甲酯胁迫的转录表达情况等进行了表征。基因结构分析表明同一亚类中各成员的基因结构特征相似;通过系统进化分析,将6个丹参BZR基因被分为两类;保守基序分析表明SmBZR蛋白的N末端均含有BZR基因家族的特征性序列;顺式作用元件分析表明SmBZR基因的上游序列含有丰富的与光响应、逆境胁迫响应,植物激素响应和生长发育相关的元件;基于已发表的转录组数据对丹参BZR基因的表达情况分析表明它们可能受茉莉酸甲酯调控。本研究为深入了解丹参BZR基因家族提供了一定的参考价值。Brassinosteroids(BR)is a kind of steroid hormone in plants.In addition to having growth regulating roles,it also has stress resistance functions.BZR(BRASSINAZOLE-RESISTANT)transcription factor is a regulator in BR signal transduction pathway,which plays a vital role in plant growth and resistance to environmental stimulations.In this study,the whole genome of Salvia miltiorrhiza Bunge member BZR was identified and 6members were identified.After a series of bioinformatics analyses,the gene structure,evolutionary relationship,conservative motifs,cis-acting elements and the transcriptional expression of MeJA(methyl jasmonate)stress were characterized.Gene structure analysis showed that the gene structure characteristics of each member of the same subclass were similar;through systematic evolution analysis,the 6 Sm BZR genes are divided into two categories;the conservative motif analysis showed that the N-terminal of SmBZR protein contained characteristic sequence of BZR gene family;from the analysis of cis-acting elements,it is known that the upstream sequence of the Sm BZR gene is rich in elements related to light response,stress response,plant hormone response and growth and development;analysis of the expression of BZR genes based on published transcriptome data indicates that they may be regulated by MeJA.This study provides a certain reference value for in-depth understanding of the BZR gene family of S.miltiorrhiza.

关 键 词：丹参 BZR基因 表达分析 生物信息学 

分 类 号：S567.53[农业科学—中草药栽培]