健脾止动汤对慢性束缚应激抽动障碍大鼠前额叶皮质神经元及神经营养因子的影响  

Effect of Jianpi Zhidong Decoction on neuronal and neurotrophic factors in prefrontal cortex of tic disorder rats with chronic restraint stress

作　　者：白雪[1] 张宏贤 周倩倩 马宁 闫文宏 张晶晶[1] 崔霞 BAI Xue;ZHANG Hongxian;ZHOU Qianqian;MA Ning;YAN Wenhong;ZHANG Jingjing;CUI Xia(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,100029 [2]南通市中医院儿科 [3]北京中医药大学第三附属医院儿科

出　　处：《环球中医药》2022年第6期991-995,共5页Global Traditional Chinese Medicine

基　　金：北京市自然基金项目(7182101);2017中医优秀人才项目(201724)。

摘　　要：目的探讨健脾止动汤对慢性束缚应激抽动障碍大鼠大脑皮质区神经元及神经营养因子水平的影响。方法将48只3周龄雄性SD大鼠随机分为空白组12只、造模组36只。空白组予生理盐水[1 mL/(kg·d)]腹腔注射7天;模型组予IDPN[250 mg/kg,1 mL/(kg·d)]腹腔注射7天,同时慢性束缚21天,造模成功后随机分为模型组、硫必利组、健脾止动汤组。空白组、模型组予生理盐水灌胃,硫必利组予盐酸硫必利混悬液灌胃(1 mL药液中约含2.1 mg原药),中药组予健脾止动汤溶液灌胃(1 mL药液中约含1.6 g生药),灌胃体积1 mL/100 g,每天灌胃1次,连续4周。末次用药后,对大鼠断头取脑,留取标本。尼氏染色法观察大鼠前额叶皮质区神经元尼氏小体改变,酶联免疫吸附法检测前额叶皮质中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)和神经生长因子(nerve growth factor,NGF)的含量。结果与空白组相比,模型组前额叶皮质中尼氏小体的数量显著减少,IGF-1含量显著降低;与模型组相比,硫必利组IGF-1含量显著升高,健脾止动汤组尼氏小体显著增多,BDNF、IGF-1、NGF含量显著升高。结论健脾止动汤具有神经保护作用,可能与提高神经营养因子的表达有关。Objective To investigate the effect of Jianpi Zhidong Decoction(JPZDD)on the neuronal and the expressions of neurotrophic factors in prefrontal cortex of Tic Disorder(TD)rats with chronic restraint stress.Methods 483-week-old male SD rats were randomly divided into the blank control group(n=12)and the model group(n=36).The blank control group rats were intraperitoneally injected with normal saline[1 mL/(kg·d)]for consecutive 7 days;the model group rats were intraperitoneally injected with IDPN[250 mg/kg,1 mL/(kg·d)]for consecutive 7 days,meantime they were given chronic restraint until the 21st day.After successful modeling,the model group rats were randomly divided into the TD model group,the Tiapride group and the JPZDD group,with 12 rats in each group.The Blank control group rats and TD model group rats were administrated with normal saline,the Tiapride group rats were administrated with Tiapride(1 mL solution containing approximately 2.1 mg of Tripride),and the JPZDD group rats were administrated with JPZDD(1 mL solution containing approximately 1.6 g of herb),1 mL/100 g,once a day for consecutive 4 weeks.The rats were sacrificed after the last behavioral test,the brains were taken.Nissl staining was used to observe the number of nissl bodies in the prefrontal contex.The contents of brain-derived neurotrophic factor(BDNF),nerve growth factor(NGF)and insulin-like growth factor-1(IGF-1)in prefrontal cortex were assayed by ELISA.Results Compared with the blank control group,the number of nissl bodies in the model group were decreased significantly and the IGF-1 levels were decreased significantly compared with the model group,the IGF-1 levels were increased significantly in the Tiapride group,the numbers of nissl bodies increased significantly,the levels of BDNF,IGF-1 and NGF were increased significantly in the JPZDD group.Conclusion Jianpi Zhidong Decoction has neuroprotective effects possibly through elevating the expressions of neurotrophic factors in rat brains.

关 键 词：健脾止动汤 多发性抽动症 脑源性神经营养因子 神经生长因子 胰岛素样生长因子-1 

分 类 号：R285.5[医药卫生—中药学]