不同吸入时间对成年大鼠七氟烷最低肺泡有效浓度的影响  

Effects of different inhalation time on minimum alveolar concentration of sevoflurane in adult rats

作　　者：周涛[1] 曹锐 张敏[1] 林芷萱 杨小霖[2] Zhou Tao;Cao Rui;Zhang Min;Lin Zhixuan;Yang Xiaolin(Department of Anesthesiology,Clinical School,North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院临床学院麻醉学系,南充637000 [2]川北医学院附属医院麻醉科,南充637000

出　　处：《中华麻醉学杂志》2022年第3期302-306,共5页Chinese Journal of Anesthesiology

基　　金：四川省医学科研课题计划(S21014);南充市校地合作项目(19SXHZ0151)。

摘　　要：目的评价吸入时间对成年大鼠七氟烷最低肺泡有效浓度(MAC)的影响。方法SPF级健康成年SD大鼠200只,8~10周龄,体重200~260 g,雌雄各半,采用随机数字表法分为2组(n=100):七氟烷吸入1 h组和20 min组,每组再分为10个亚组(n=10),预设起始浓度为1.50%,相邻浓度比值r为1.08,通过夹尾刺激判定各亚组麻醉效果,1 min内出现体动反应为阳性,未出现为阴性。采用Bliss法计算2组七氟烷MAC、EC95及其95%置信区间。结果吸入1 h组七氟烷MAC、EC95及其95%置信区间分别为2.09%(1.98%~2.20%)和2.75%(2.56%~3.04%),吸入20 min组七氟烷MAC、EC95及其95%置信区间分别为2.35%(2.22%~2.49%)和3.10%(2.87%~3.45%),差异有统计学意义(P<0.05)。结论吸入七氟烷1 h成年大鼠MAC较吸入20 min时降低。Objective To evaluate the effects of different inhalation time on the minimum alveolar concentration(MAC)of sevoflurane in adult rats.Methods Two hundred SPF healthy adult Sprague-Dawley rats(half male,half female),aged 8-10 weeks,weighing 200-260 g,were divided into 2 groups using a random number table method:inhalation of sevoflurane for 1 h group and inhalation of sevoflurane for 20 min group,with 100 rats in each group.Each group was subdivided into 10 subgroups with 10 rats in each subgroup,the initial concentration was preset at 1.50%,and the ratio between two successive concentrations r was 1.08.The tail clamping stimulus was applied to evaluate the efficacy of anesthesia in each subgroup,a positive response was defined as a body movement occurred within 1 min after tail clamping stimulus,and the response was defined as negative when no body movement occurred within 1 min after tail clamping.The Bliss method was used to calculate the MAC,EC95 and 95%confidence interval(CI)of sevoflurane.Results The MAC and EC95(95%CI)of sevoflurane were 2.09%(1.98%-2.20%)and 2.75%(2.56%-3.04%),respectively,in inhalation of sevoflurane for 1 h group,and 2.35%(2.22%-2.49%)and 3.10%(2.87%-3.45%),respectively,in inhalation of sevoflurane for 20 min group(P<0.05).Conclusions The MAC of sevoflurane in adult rats inhaled sevoflurane for 1 h is decreased than that inhaled for 20 min.

关 键 词：麻醉药 吸入 剂量效应关系 药物 

分 类 号：R614[医药卫生—麻醉学]