利用AhMITE分子标记对花生杂交F_(1)代的真伪鉴定  

Identification of F_(1) Generation Peanut Hybrids Using AhMITE Molecular Markers

作　　者：徐彪 李玉发[2] 牛海龙[2] 李伟堂[2] 刘红欣[2] 王伟[2] 吴松权[1] 何中国[2] XU Biao;LI Yu-fa;NIU Hai-long(Agricultural College of Yanbian University,Yanji,Jilin 133000;Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136110)

机构地区：[1]延边大学农学院,吉林延吉133000 [2]吉林省农业科学院,吉林公主岭136110

出　　处：《安徽农业科学》2022年第11期94-97,共4页Journal of Anhui Agricultural Sciences

基　　金：吉林省农业科技创新工程人才基金-研究生基金(C02103506);吉林省农业农村厅-现代农业产业技术示范推广(202106)。

摘　　要：[目的]提高花生育种效率。[方法]从10对AhMITE引物中筛选出对杂交亲本具有差异条带的标记引物,分别对10个花生杂交组合197个F_(1)代单株进行真伪杂种鉴定。[结果]根据PCR扩增的父母本带型,共鉴定出75个真杂种单株,真杂种比例为38.02%。[结论]利用AhMITE分子标记可以快速、准确地对花生杂交F_(1)代进行真伪鉴定,为进一步使用AhMITE分子标记技术对吉林省花生新品种选育及花生种质资源遗传多样性分析提供技术支撑。[Objective]In order to improve the efficiency of flower breeding.[Method]Screened from 10 pairs of AhMITE primers for the marker primers with different bands in the hybrid parents,197 F_(1) single plants of 10 peanut cross combinations were used to identify the true and false hybrids.[Result]According to the parental band type amplified by PCR,a total of 75 true hybrid single plants were identified,and the true hybrid ratio was 38.02%.[Conclusion]The AhMITE molecular marker can be used to quickly and accurately identify the authenticity of the peanut hybrid F_(1) generation.It is expected to provide technical support for the further use of the AhMITE molecular marker technology in the selection of new peanut varieties in Jilin Province and the analysis of the genetic diversity of peanut germplasm resources.

关 键 词：花生 F_(1)真伪鉴定 AhMITE标记 

分 类 号：S565.2[农业科学—作物学]