基于扬麦158/CI12633重组自交系群体的籽粒千粒重QTL分析  

Analysis of QTLs for the Thousand-gran Weight in a Recombiannt Inbred Lines Population from Yangmai158/CI12633

作　　者：郭元世[1] 梅佳[1] 罗德祥[1] 涂军[1] 陆健康[1] 厉婕 侯运章 吕乐城 周淼平[2] GUO Yuan-shi;MEI Jia;LUO De-xiang(Jiangsu Choosan Co.,Ltd.,Nanjing,Jiangsu 221500)

机构地区：[1]江苏中江种业股份有限公司,江苏南京221500 [2]江苏省农业科学院粮食作物研究所,江苏南京210014

出　　处：《安徽农业科学》2022年第11期98-100,105,共4页Journal of Anhui Agricultural Sciences

摘　　要：利用高产广适亲本扬麦158和抗纹枯病种质资源CI12633构建的重组自交系(RIL)群体为研究对象,采用ddRAD-seq技术开发SNP标记并构建遗传连锁图,结合RIL群体2018—2020年的千粒重表型数据进行千粒重相关QTL的定位。结果表明:RIL群体的千粒重存在超双亲分离,且基本符合正态分布,RIL群体家系间及年度间的差异均达极显著水平;2018—2020年共检测到5个QTL位点,分别位于1A、4A、6A、6B和7D染色体,单个QTL可解释9.70%~21.80%的表型变异,其中位于4A和6B染色体的2个QTL位点可在不同年份重复检测到,可能为主效QTL,可用于分子标记辅助选择育种。The objectives of the present study were to detect QTLs of thousand grain weight using ddRAD-seq technology to develop SNP markers and construct genetic linkage map in a recombinant inbred lines population derived from a cross between Yangmai158 and CI12633,and evaluated for thousand grain weight at three years under organic management.The results showed that the thousand grain weight of the recombinant inbred lines has super parental separation,and basically conforms to the normal distribution.The differences between families and years of RIL population have reached a very significant level.Five QTLs for TKW were mapped on chromosomes 1A,4A,6A,6B and 7D.QTL showing proportions of 9.70%-21.80%of phenotypic variance explained in at least two environments were located on chromosomes 4A and 6B,which may prove useful for MAS for improvement of the thousand grain weight in bread wheat.

关 键 词：小麦 重组自交系 千粒重 QTL 

分 类 号：S512.1[农业科学—作物学]