旱柳、杞柳ACO基因家族的全基因组鉴定及表达分析  

Genome-wide identification and expression analysis of ACO gene family in Salix matsudana and Salix integra

作　　者：冯志聪 邹航 果弘毅 钟非[1] 陈艳红[1] 余春梅[1] 连博琳 张健 刘国元 FENG Zhicong;ZOU Hang;GUO Hongyi;ZHONG Fei;CHEN Yanhong;YU Chunmei;LIAN Bolin;ZHANG Jian;LIU Guoyuan(Key Laboratory of Landscape Plant Genetics and Breeding in Nantong/College of Life Science,Nantong University,Nantong 226019,China)

机构地区：[1]南通大学生命科学学院/南通市观赏植物遗传育种重点实验室,江苏南通226019

出　　处：《扬州大学学报（农业与生命科学版）》2022年第2期10-19,共10页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31971681);南通市基础科学研究项目(JC2020157);江苏省大学生创新创业训练计划项目(202110304020Z)。

摘　　要：1-氨基环丙烷-1-羧酸氧化酶(1-aminocyclopropane-1-carboxylic acid oxidase,ACO)是植物中乙烯生物合成途径的限速酶。以ACO蛋白的保守结构域特征为基础,在旱柳(Salix matsudana)和杞柳(Salix integra)的全基因组测序结果中分别鉴定出42和33个ACO蛋白;并运用系列生物信息学方法分析其进化关系、基因结构、理化性质与表达模式等。结果表明:该家族成员可分为10个组,每组的保守基序特征与进化树基本一致。ACO基因含有3~6个外显子,编码238~438个氨基酸,蛋白质理论PI值在5.03~9.52之间,分子量为9.794~48.537 ku;染色体定位显示旱柳ACO基因分布在19条染色体上,部分基因串联重复形成基因簇。热图结果发现ACO基因家族成员在速生性强、弱品系中的表达水平具有差异性。1-aminocyclopropane-1-carboxylic oxidase(ACO)is a rate-limiting enzyme in the ethylene biosynthesis pathway in plants.In our study,based on the conserved domain characteristics of ACO protein,42 and 33 ACO proteins were identified in the whole genome sequencing of Salix matsudana and S.integra,respectively.A series of bioinformatics methods were used to analyze their evolutionary relationship,gene structure,physicochemical properties and expression patterns.The results showed that the members of this family can be divided into 10 groups,and the conserved motif characteristics of each group were consistent with those of the evolutionary tree.The ACO genes contain 3-6 coding sequences,encoding 238-438 amino acids.The theoretical PI values of these proteins ranged from 5.03 to 9.52,and the molecular weight ranged from 9.794 to 48.537 ku.Chromosome mapping results showed that the ACO genes of S.matsudana were distributed on 19 chromosomes,and some genes were repeated in tandem to form gene clusters.The results of heat map showed that the ACO gene family members had different expression levels in the strong and weak fast-growing strains.

关 键 词：柳属 旱柳 杞柳 速生性 ACO基因家族 生物信息学 

分 类 号：S188[农业科学—农业基础科学]