姜黄素改善小鼠急性溶血性贫血的作用及机制研究  

The effect and mechanism of curcumin on alleviateing acute hemolytic anemia in mice

作　　者：吴凡 杨蕾 张毅 魏子辰 郁多男 WU Fan;YANG Lei;ZHANG Yi;WEI Ziehen;YU Duonan(Jiangsu Key Laboratory of Experimental&Translational Non-coding RNA Research/College of Medicine,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学医学院/江苏省非编码RNA基础与临床转化重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2022年第2期78-85,91,共9页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金面上项目(81870096);江苏省研究生培养创新工程与研究生科研创新项目(KYCX20-2994)。

摘　　要：为探究姜黄素(CUR)能否改善小鼠急性溶血性贫血以及其保护红细胞的作用机制,首先采用腹腔注射苯肼的方法构建小鼠急性溶血性贫血模型;经姜黄素给药后,外周血涂片和瑞氏-姬姆萨染色观察小鼠红细胞形态;全血细胞分析仪检测血常规指标;流式细胞术检测小鼠外周血、骨髓、脾脏红细胞成熟度及ROS水平;随后利用Western blot和qRT-PCR检测脾脏有核红细胞中Nrf2及下游抗氧化元件的表达水平。结果表明:姜黄素治疗组外周血红细胞恢复得更好更快,脾脏及骨髓代偿造血明显增强,且各阶段红细胞ROS水平显著降低。同时,Nrf2蛋白水平及下游部分抗氧化酶的表达均有所上升。这一研究证实,姜黄素可通过上调Nrf2及下游抗氧化酶来清除红细胞ROS,从而改善小鼠急性溶血性贫血。To explore whether curcumin(CUR) improves acute hemolytic anemia in mice and its mechanism of protecting erythrocytes against oxidative stress, we established a mouse model of acute hemolytic anemia by intraperitoneal injection of phenylhydrazine(PHZ). Blood smear and Wright-Giemsa staining were used to observe the morphology of red blood cells;complete blood counts were determined using a hematology analyzer;flow cytometry was used to examine developmental stage and ROS levels of erythrocytes in peripheral blood, bone marrow and spleen;expression levels of Nrf2 and downstream antioxidant molecules in nucleated erythroid cells were detected by qRT-PCR and Western blot. Results showed that during the recovery phase of PHZ-induced acute anemia, the curcumin-treated mice exhibited a better recovery in peripheral blood, faster emergence of erythroid precursors, less ROS in erythroid cells from bone marrow and spleen. This study confirmed that the curcumin protects erythrocytes against oxidant stress, and thus alleviates acute hemolytic anemia in mice by upregulating expression levels of Nrf2 and downstream antioxidant enzymes.

关 键 词：姜黄素 溶血性贫血 红细胞 NRF2 抗氧化 

分 类 号：R285[医药卫生—中药学]