基于网络药理学探究丹参饮治疗冠状动脉粥样硬化性心脏病的潜在作用机制  

Exploring the potential mechanism of Danshen Yin in the treatment of coronary heart disease based on network pharmacology

作　　者：常鑫迪 朱明军[2] 王永霞[2] 李彬[2] 于瑞[2] 卫靖靖 郑超楠 CHANG Xindi

机构地区：[1]河南中医药大学,河南郑州450000 [2]河南中医药大学第一附属医院,河南郑州450000

出　　处：《中医临床研究》2022年第7期1-7,共7页Clinical Journal Of Chinese Medicine

基　　金：国家重点研发计划重点专项课题(2019YFC1710000、2019YFC 1710003);国家重点基础研究发展计划项目(2015CB554401);国家中医药管理局中医药循证能力建设项目(2019XZZX-XXG003);河南省创新型科技团队(C20130050)。

摘　　要：目的:采用网络药理学筛选丹参饮中的活性成分和作用靶点,分析治疗冠状动脉粥样硬化性心脏病(简称冠心病的潜在作用机制。方法:通过检索中药系统药理学数据库和分析平台(TCMSP)、PubChem、Swiss及UniProt数据库筛选丹参饮的有效成分及作用靶点,使用GeneCards和OMIM获得疾病的致病靶点,在Veney 2.1.0数据库中将药物的作用靶点和疾病的致病靶点取交集得到核心靶点。使用String数据库构建靶点蛋白质-蛋白质相互作用网络,筛选连接度排名前10位的靶点蛋白,借助网络拓扑属性分析软件Cytoscape 3.7.2构建药物活性成分-疾病-靶点网络。利用DAVID对靶点基因本体论(GO)功能和京都基因与基因组百科全书(KEGG)中代谢通路进行富集分析。结果:从丹参饮中共筛选出活性成分78个,与冠心病相关的共同作用靶点169个,核心靶点主要包括肿瘤坏死因子、白细胞介素-6、血清白蛋白、丝氨酸/苏氨酸蛋白激酶、血管内皮生长因子A、丝裂原活化蛋白激酶1;GO生物过程结果显示与RNA聚合酶Ⅱ启动子转录的正调控、信号转导、质膜、ATP结合、蛋白同源二聚体化活性等相关;KEGG通路富集分析结果显示主要涉及磷脂酰肌醇3激酶-蛋白激酶B、肿瘤坏死因子、丝裂原活化蛋白激酶、缺氧诱导因子-1、叉头框O等信号通路。结论:丹参饮通过多种药物活性成分及靶点效应有效干预冠心病,分析成分、靶点及通路的相互关系可为进一步实验及临床研究提供基础。Objective:To screen the active components and targets of Danshen Yin(丹参饮)by network pharmacology,and to analyze the potential mechanism in the treatment of coronary heart disease.Methods:Through TCMSP,PubChem,Swiss,UniProt databases,all chemical constituents of Danshen Yin were searched to screen out the effective targets.Disease targets were collected through OMIM and GeneCards databases.In Veney 2.1.0 database,the effective target of medicine and the pathogenic target of disease were intersected to obtain the core target.Target protein-protein interaction network was established by String database,and top 10 target proteins in the list of connectivity were screened.Network topology attribute analysis software Cytoscape 3.7.2 was utilized to construct the active componentsdisease-targets network.The GO function of target and metabolic pathways in KEGG were enriched and analyzed by DAVID.Results:78 active components and 169 common targets related to CHD were screened from Danshen Yin.The core targets included TNF,IL6,ALB,AKT1,VEGFA and MAPK1.The results of GO biological process showed that it was related to the positive regulation of RNA polymeraseⅡpromoter,signal transduction,plasma membrane,ATP binding,protein homodimerization activity,etc.KEGG pathway enrichment analysis showed that it mainly involved PI3K-Akt,TNF,MAPK,HIF-1,FoxO and other signal pathways.Conclusion:Danshen Yin can effectively interfere with coronary heart disease through a variety of drug active components and target effects,and the analysis of the relationship among components,targets and pathways can provide a basis for further experiment and clinical research.

关 键 词：丹参饮 冠状动脉粥样硬化性心脏病 作用机制 网络药理学 

分 类 号：R256.2[医药卫生—中医内科学]