Diagnosis of cytomegalovirus encephalitis using metagenomic nextgeneration sequencing of blood and cerebrospinal fluid:A case report  

作　　者：Chang-Qing Xu Xia-Ling Chen Dong-Sheng Zhang Jia-Wei Wang Hong Yuan Wei-Fan Chen Han Xia Zhong-Yin Zhang Fu-Hua Peng 

机构地区：[1]Department of Neurology,Dongguan Kanghua Hospital,Dongguan 523080,Guangdong Province,China [2]Department of Scientific Affairs,Hugobiotech Co.,Ltd.,Beijing 100176,China [3]Department of Neurology,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,Guangdong Province,China

出　　处：《World Journal of Clinical Cases》2022年第14期4601-4607,共7页世界临床病例杂志

摘　　要：BACKGROUND Cytomegalovirus(CMV)infections in the population are mostly subclinical,inapparent,or latent.However,it is rare in brain tissue.Most reported CMV encephalitis cases were patients with immunodeficiency.The diagnosis and detection rate of CMV encephalitis in patients with normal immune function needs to be further improved.CASE SUMMARY An 86-year-old male was admitted due to a sudden onset of unconsciousness for 3 h.The patient developed status epilepticus and was relieved after antiepileptic treatment.Encephalitis was considered due to the high signals of diffusionweighted imaging sequences in the right central region by magnetic resonance imaging.Metagenomic next-generation sequencing(mNGS)of blood and cerebrospinal fluid revealed CMV,with unique reads number being 614 and 1,respectively.Simultaneous quantitative PCR results showed CMV positive in blood samples and negative in cerebrospinal fluid samples.The patient was finally diagnosed as CMV encephalitis with status epilepticus.After the antiviral,hormonal,andγ-globulin pulse therapy,the patient’s condition improved,and he was finally discharged.CONCLUSION mNGS could be a reliable approach for the diagnosis of CMV encephalitis,with high efficiency,sensitivity,and specificity.

关 键 词：Cytomegalovirus encephalitis Metagenomic next-generation sequencing Quantitative real-time PCR DIAGNOSIS Case report 

分 类 号：R512.3[医药卫生—内科学]