Potential of Antibody-Dependent Cellular Cytotoxicity in Acute and Recovery Phases of SARS-CoV-2 Infection  

作　　者：Tingting Cui Mingzhu Huang Xiaoling Su Zhengfang Lin Jiaying Zhong Xiaoyun Yang Zhongfang Wang 

机构地区：[1]The State Key Laboratory of Respiratory Disease&National Clinical Research Center for Respiratory Disease,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou Medical University,Guangzhou 510180,China

出　　处：《Infectious Diseases & Immunity》2022年第2期74-82,共9页感染性疾病与免疫（英文）

摘　　要：Background:Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has caused a global pandemic that has resulted in millions of casualties.Although researchers have reported the existence of neutralizing antibodies and viral T cell immunity against SARS-CoV-2,little is known about the presence of antibody-dependent cellular cytotoxicity(ADCC)and its role in combating SARS-CoV-2 infection.Methods:Nineteen acute COVID-19 patients at the First Affiliated Hospital of Guangzhou Medical University from January to February,2020 and 55 recovery COVID-19 patients at the Second Peoples Hospital of Changde City from February,2020 to February,2021 were recruited in this study.Longitudinal plasma samples were collected.A virus-specific ADCC assay was performed to study the COVID-19 plasma samples.The correlations between ADCC and total IgG titer,including anti-RBD,anti-N,and neutralizing antibody titer were analyzed.Results:A high level of ADCC with 0.86%of IFN-g+CD107a+NK cells induced by anti RBD antibodies and with 0.54%of IFN-g+CD107a+NK cells induced by anti N antibodies was observed.This activity peaked at 3 weeks after disease onset with 1.16%and 0.63%of IFN-g+CD107a+NK cells induced by anti RBD and anti N antibodies respectively,declined to 0.32%and 0.32%of IFN-g+CD107a+NK cells respectively after more than 2 months,and persisted for 12 months after disease onset.The ADCC did not aggravate the severity of COVID-19 in terms of sequential organ failure assessment,although ADCC decreased with the age of COVID-19 patients.Interestingly,ADCC response is not correlated with neutralizing antibody titer or total IgG titers against S protein RBD and N protein in acute patients.ADCC in recovered patients showed a significant correlation with anti RBD IgG titer(R2=0.33,P<0.001).Conclusion:Antibodies from COVID-19 patients against the N protein and S protein RBD domains could stimulate high levels of ADCC response.Our results provide evidence that vaccination should not only focus on neutralizing antibodies but also binding a

关 键 词：Antibody-dependent cell cytotoxicity Aged immunity Anti-viral immunity SARS-CoV-2 SEVERITY 

分 类 号：R563.1[医药卫生—呼吸系统]