药典法测定黄芪药材黄芪甲苷含量方法的商榷  

Discussion on Pharmacopoeia Method for Determination of Astragaloside Ⅳ in Astragalus

作　　者：肖柳君 吕尚 魏惠珍[1,2] 金浩鑫[1] 杨磊 丁若雯 丁平平 饶毅[1,2] XIAO Liu-jun;LV Shang;WEI Hui-zhen;JIN Hao-xin;YANG Lei;DING Ruo-wen;DING Ping-ping;RAO Yi(Jiangxi University of Chinese Medicine,Nanchang 330004,China;The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine,Nanchang 330006,China)

机构地区：[1]江西中医药大学,南昌330004 [2]中药固体制剂制造技术国家工程研究中心,南昌330006

出　　处：《江西中医药大学学报》2022年第2期84-87,共4页Journal of Jiangxi University of Chinese Medicine

摘　　要：目的:探讨黄芪中黄芪甲苷含量测定方法可行性。方法:采用中性氧化铝柱层析方法与药典法对黄芪药材进行处理,对两种方法所测不同批次黄芪中黄芪甲苷含量数据进行比较。结果:采用中性氧化铝柱层析方法处理,黄芪甲苷在0.2343~1.1715 mg/mL呈良好的线性关系,平均回收率为100.56%(RSD=3.28%),中性氧化铝柱层析法、《中国药典》法测定黄芪药材中黄芪甲苷含量分别为0.0740%,0.1005%,0.1302%,0.1113%,0.1520%,0.0998%,0.0793%,0.1303%和0.0694%,0.0860%,0.0854%,0.0757%,0.1054%,0.0698%,0.0402%,0.0720%。结论:采用中性氧化铝柱层析方法对黄芪药材进行处理,较药典法操作起来更简便、重复性更好。该方法可以为快速、准确测定黄芪甲苷含量提供参考。Objective:To study the feasibility of determination of astragaloside Ⅳ in Astragali Radix.Method:Neutral alumina column chromatography and Chinese Pharmacopoeia were used to treat Astragalus membranaceus,and the data of astragaloside Ⅳ in different batches of Astragalus membranaceus determined by the two methods were compared.Results:Treatment with neutral alumina column chromatography,Astragaloside Ⅳ showed a good linear relationship in the range of 0.2343~1.1715 mg/mL.The average recovery rate of Astragaloside Ⅳ was 100.56%(RSD=3.28%).The contents of astragaloside Ⅳ in Astragali Radix determined by Neutral alumina column chromatography and pharmacopoeia method were 0.0740%,0.1005%,0.1302%,0.1113%,0.1520%,0.0998%,0.0793%,0.1303%;0.0694%,0.0860%,0.0854%,0.0757%,0.1054%,0.0698%,0.0402%,0.0720%.Conclusion:Treatment of Astragalus membranaceus by neutral alumina column chromatography is simpler and more reproducible than pharmacopeia.This method can provide a reference for rapid and accurate determination of astragaloside IV.

关 键 词：黄芪 黄芪甲苷 含量测定 D101大孔吸附树脂 中性氧化铝 

分 类 号：R284.1[医药卫生—中药学]