生花和不生花泡菜盐水真菌群落结构的对比  

A comparative study on the fungal community structure between pellicle and non-pellicle brines of Chongqing Paocai

作　　者：杨吉霞[1,3] 曾祥平 李玥梦 李舒婷 贺稚非 YANG Jixia;ZENG Xiangping;LI Yuemeng;LI Shuting;HE Zhifei(College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Academy of Metrology and Quality Inspection of First Branch,Chongqing 402260,China;Chongqing Key Laboratory of Specialty Food Co-Built by Sichuan and Chongqing,Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]重庆市计量质量检测研究院第一分院,重庆402260 [3]川渝共建特色食品重庆市重点实验室,重庆400715

出　　处：《食品与发酵工业》2022年第11期58-64,共7页Food and Fermentation Industries

基　　金：国家自然科学基金青年基金项目(Grant No.31801529);重庆市基础科学与前沿技术研究项目(cstc2017jcyjAX0249);西南大学中央高校基本科研业务费项目(XDJK2018C070,XDJK2015C135,SWU113035);四川省教育厅川菜发展研究中心科研项目(CC21Z30)。

摘　　要：重庆地区有制作和食用家庭自制泡菜的传统,生花是泡菜盐水的常见现象。为了更深入地认识生花相关的真菌群落结构信息,该研究采用Illumina MiSeq技术对生花(P组)和不生花(NP组)盐水样品的ITS1基因进行测序,解析两组的真菌群落结构及其差异。P组样品中主要的属包括:Pichia(85.22%)、Candida(2.07%)、Aspergillus(1.54%)、Fusarium(1.25%);NP组包括:Pichia(46.04%)、Debaryomyces(21.75%)、Aspergillus(9.57%)、Kazachstania(5.31%)、Simplicillium(2.72%)、Penicillium(1.43%)、Wickerhamomyces(1.32%)、Fusarium(1.15%)、Candida(1.04%)、Schwanniomyces(1.03%)。P组真菌的平均拷贝数几乎是NP组的20倍。Kruskal Wallis检验发现两组的Debaryomyces和Kazachstania相对丰度存在显著性差异。该研究阐述了与生花现象相关的真菌群落结构信息,有助于防控生花现象。Preparing and consuming home-made Paocai is a tradition in Chongqing.The pellicle(biofilm)formation in Paocai brines is a common problem during fermentation.In order to study the fungi related to pellicle,Illumina MiSeq sequencing was used to sequence the ITS1 genes of the Paocai brine samples with(P group)and without(NP group)pellicle,the differences in fungal community structures between the two groups were investigated.The major genera of group P included Pichia(85.22%),Candida(2.07%),Aspergillus(1.54%)and Fusarium(1.25%).The major genera of NP group included Pichia(46.04%),Debaryomyces(21.75%),Aspergillus(9.57%),Kazachstania(5.31%),Simplicillium(2.72%),Penicillium(1.43%),Wickerhamomyces(1.32%),Fusarium(1.15%),Candida(1.04%)and Schwanniomyces(1.03%).The average biomass of fungi in group P was almost 20 times than that of group NP.Significant differences were observed in the relative abundance of genus Debaryomyces and Kazachstania by Kruskal Wallis test.This study demonstrates the fungal community structure information related to pellicle phenomenon,which is helpful for preventing and controlling pellicle in home-made Paocai.

关 键 词：重庆泡菜 生花现象 Illumina MiSeq测序 真菌群落结构 

分 类 号：TS255.54[轻工技术与工程—农产品加工及贮藏工程]