《中华人民共和国药典》2020年版中防己薄层色谱鉴别研究  

Investigation on the Identification of Stephaniae Tetrandrae in the Chinese Pharmacopoeia 2020 by TLC

作　　者：曹朴琼 陶明宝 杨深应 赵霞 陈琪 顾小迪 Cao Puqiong;Tao Mingbao;Yang Shenying;Zhao Xia;Chen Qi;Gu Xiaodi(Baoshan Food and Drug Testing Center,Baoshan 678000,China;Baoshan College of Traditional Chinese Medicine,Baoshan 678000,China)

机构地区：[1]保山市食品药品检验检测中心,云南保山678000 [2]保山中医药高等专科学校,云南保山678000

出　　处：《云南化工》2022年第5期44-46,共3页Yunnan Chemical Technology

摘　　要：(目的)探讨完善《中华人民共和国药典》2020年版中防己的薄层色谱鉴别项。(方法)以防己对照药材、粉防己碱、防己诺林碱为对照,采用硅胶GF254薄层板,以乙酸乙酯-丙酮-甲醇-5%氨水(体积比7∶1∶0.7∶0.3)为展开剂,置紫外光灯(254 nm)下检视。(结果)展开剂可选用低毒的乙酸乙酯代替三氯甲烷,防己对照药材色谱斑点清晰,分离度好,比原标准显示出更多鉴别信息。(结论)为《中华人民共和国药典》2020年版中防己质量标准的进一步提高完善提供参考依据。(Objective)To discuss and improve the TLC identification of Stephaniae tetrandrae in the Chinese pharmacopoeia 2020.(Method)The reference substances of Stephaniae tetrandrae,tetrandrine and Fangchinoline were used,and the silica gel GF254 thin-layer plate was adopted.The mixed solution of ethyl acetate,acetone,methanol,5%ammonia(7∶1∶0.7∶0.3)was used as the developing solvent,examined under the UV light(254nm).(Result)low-toxic ethyl acetate was used as the developing solvent instead of chloroform,The TLC identification method of Stephaniae tetrandrae reference drug had the good resolution,the clearly colored slots were obtained,more informatic characteristic bands than the original standard were get.(Conclusion)The study can provide a reference for the further improvement of the drug standard of Stephaniae tetrandrae in the Chinese Pharmacopoeia 2020.

关 键 词：防己 粉防己碱 防己诺林碱 薄层色谱鉴别 

分 类 号：R921.2[医药卫生—药学]