珍珠粉提取物对人A375黑色素瘤细胞黑素合成的影响  

Effect of Pearl Powder Extract on Melanogenesis in A375 Melanoma Cells

作　　者：陈怡 罗文[1] CHEN Yi;LUO Wen(School of Life Sciences,Shaoxing University,Shaoxing,Zhejiang 312000)

机构地区：[1]绍兴文理学院生命科学学院,浙江绍兴312000

出　　处：《绍兴文理学院学报》2022年第4期51-57,共7页Journal of Shaoxing University

基　　金：浙江省科技特派员团队项目“珍珠品质提升及产业化”.

摘　　要：目的:以人A375黑色素瘤细胞为模式细胞,浅探珍珠粉中起美白作用的成分及作用机理.方法:制备珍珠粉总提取物和蛋白提取物,作用于经紫外线照射过的A375细胞中,分别采用MTT法测定细胞增殖活性,多巴氧化法测定酪氨酸酶活性,NaOH裂解法测定黑素合成含量.结果:在紫外线照射下,A375细胞内酪氨酸酶活性和黑色素含量均显著上升(P<0.05);而珍珠粉总提取物能够增加A375细胞酪氨酸酶的活性(P<0.05),但不影响细胞内黑色素的含量;珍珠粉蛋白提取物不改变细胞酪氨酸酶的活性,却能抑制细胞内黑色素的产生(P<0.05),这说明珍珠粉总提取物能够保护A375黑色素瘤细胞免受紫外线的伤害,其蛋白提取物在紫外线作用下还具有美白作用.结论:珍珠粉中起美白作用的物质主要存在于蛋白质中,珍珠粉蛋白提取物通过调控酪氨酸酶的活性控制黑素合成量达到美白的效果.Objective:To investigate the whitening ingredients and mechanism of pearl powder,A375 melanoma cells were used as model cells.Methods:Total extract and protein extract of pearl powder were prepared and acted on UV-irradiated A375 cells.Cell proliferation activity was determined by the MTT method,tyrosinase activity by L-dopa oxidation method,and melanin synthesis content by NaOH analysis method.Results:The tyrosinase activity and melanin content in A375 cells increase significantly under UV irradiation(P<0.05).However,the total extract of pearl powder increases the activity of tyrosinase in A375 cells(P<0.05),but does not affect the content of melanin in cells.Protein extract of pearl powder does not change cell tyrosinase activity,but inhibits melanin production(P<0.05).It indicates that the total extract of pearl powder can protect A375 melanoma cells from UV damage,and its protein extract has a whitening effect under the action of UV.Conclusions:The results suggest that the substances that play a whitening role in pearl powder are mainly found in proteins,and the protein extract of pearl powder controls the amount of melanin synthesis by regulating the activity of tyrosinase to achieve the whitening effect.

关 键 词：珍珠粉提取物 A375细胞 细胞增殖 酪氨酸酶 黑色素 紫外线照射 

分 类 号：R285.5[医药卫生—中药学]