和胃利垣汤对萎缩性胃炎大鼠病理改变及白细胞介素1β、表皮生长因子、血管活性肠肽的影响  

Effects of Hewei Liyuan Decoction on Pathological Changes and Level of Interleukin 1β,Epidermal Growth Factor,Vascular Active Intestinal Peptidein Rats with Atrophic Gastritis

作　　者：董菁菁 王允野[1] 王维纲[1] 刘歌明[1] DONG Jingjing;WANG Yunye;WANG Weigang;LIU Geming(Affiliated Hospital of Jilin Medical College,Jilin 132013,China)

机构地区：[1]吉林医药学院附属医院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2022年第2期209-212,共4页Journal of Beihua University（Natural Science）

基　　金：吉林省中医药管理局中医药科技项目(2020192).

摘　　要：目的探讨和胃利垣汤对萎缩性胃炎大鼠病理改变及白细胞介素1β(IL-1β)、表皮生长因子(EGF)、血管活性肠肽(VIP)水平的影响及其作用机制.方法将36只160~180 g雄性大鼠随机分为6组,分别为空白对照组、模型组、叶酸治疗组及和胃利垣汤低剂量组、中剂量组及高剂量组.除空白对照组大鼠正常饲养不予造模外,其余大鼠在18周内造模诱导大鼠萎缩性胃炎.造模成功后治疗组分别给予叶酸(1.5 mg/kg)和胃利垣汤(0.9、1.8、3.6 g/kg)灌胃治疗12周.治疗后观察大鼠一般状态,测定胃黏膜组织IL-1β、EGF、VIP含量及各组大鼠胃窦组织病理变化.结果与空白对照组比较,模型组大鼠一般生存状况较差,摄食量减少,体质量增长较慢,HE染色镜下见胃黏膜萎缩变薄,黏膜腺体减少、排列稀疏而紊乱,伴有肠上皮化生,黏膜及黏膜下层大量炎性细胞浸润,个别可见上皮细胞轻度异性增生.与空白组大鼠胃黏膜IL-1β、EGF、VIP比较,模型组大鼠胃黏膜组织IL-1β含量显著下降,EGF、VIP显著升高(均P<0.05).经给予和胃利垣汤或叶酸灌胃治疗后IL-1β含量下降,EGF、VIP含量均有不同程度升高,其中和胃利垣汤高剂量组差异显著(P<0.05).结论和胃利垣汤可使萎缩性胃炎大鼠胃黏膜IL-1β含量下降,EGF、VIP升高,病理情况得到改善.Objective To explore the effects of Hewei Liyuan Decoction on the pathological changes and the levels of interleukin 1β(IL-1β),epidermal growth factor(EGF)and vasoactive intestinal peptide(VIP)in rats with atrophic gastritis,and its mechanism.Method 36 male rats of 160~180 g were randomly divided into 6 groups,namely the blank control group,the model group,the folic acid treatment group and the low-dose Hewei Liyuan decoction group,middle-dose group and high-dose group.Except that the rats in the blank control group were fed normally and were not modeled,the other rats were modeled to induce atrophic gastritis within 18 weeks.After the model was successfully established,the treatment group was given folic acid(1.5 mg/kg),and Hewei Liyuan decoction(0.9,1.8,3.6 g/kg)orally for 12 weeks.After treatment,the general state of the rats was observed,and the contents of IL-1β,EGF,VIP in gastric mucosa tissue and the pathological changes of gastric antrum tissue in each group were determined.Results Compared with the blank control group,the rats in the model group generally have poorer survival conditions,reduced food intake,and slower weight gain.Under HE staining microscope,gastric mucosa atrophy and thinning,mucosal glands are reduced,sparsely arranged and disordered,accompanied by Intestinal metaplasia,infiltration of a large number of inflammatory cells in the mucosa and submucosa,and mild heterogeneous proliferation of epithelial cells can be seen in some cases.Compared with IL-1β,EGF,VIP of gastric mucosa of rats in the blank group,IL-1βcontent of gastric mucosal tissue of rats in the model group decreased significantly,and the EGF and VIP increased significantly(P<0.05).After the administration of Hewei Liyuan Decoction or folic acid gavage treatment,the IL-1βcontent decreased,and EGF and VIP contents increased to varying degrees.Among them,the difference between the high-dose group and Hewei Liyuan Decoction was significant(P<0.05).Conclusion Hewei Liyuan Decoction can decrease the IL-1βcontent of the gastr

关 键 词：萎缩性胃炎 和胃利垣汤 白细胞介素1Β 表皮生长因子 血管活性肠肽水平 

分 类 号：R273[医药卫生—中西医结合]