N-α乙酰基转移酶表达对顺铂抑制人食管鳞癌ECA109细胞敏感性的影响  

Effect of N-α acetyltransferase expression on cisplatin inhibition of sensitivity of human esophageal squamous cell carcinoma ECA109 cells

作　　者：武兴刚 黄文华 董星星 赵伯文 张祥鑫 魏育涛[1,3] WU Xing-Gang;HUANG Wen-Hua;DONG Xing-Xing(Department of Cardiothoracic Surgery,the First Affiliated Hospital of Shihezi University School of Medicine,Shihezi 832000,Xinjiang,China)

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832000 [2]赣州市立医院心胸外科 [3]济宁市第一人民医院胸外科

出　　处：《中国老年学杂志》2022年第11期2741-2745,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81460059);新疆生产建设兵团卫生科技-兵团博士基金(2014BB019);新疆生产建设兵团区域创新引导计划项目(2017BA046)。

摘　　要：目的观察N-α乙酰基转移酶(Naa10)表达对顺铂(DDP)抑制人食管鳞癌ECA109细胞敏感性的影响。方法利用小干扰RNA(siRNA)构建低表达Naa10的人食管鳞癌ECA109细胞株,实验分为ECA109-siRNA-Naa10组、ECA109-siRNA-NC组(转染无效干扰片段),并设立空白ECA109细胞为ECA109-空白组。通过荧光显微镜及Western印迹法鉴定干扰效率,使用CCK-8法检测各处理组细胞经过药物处理后对DDP的敏感性。结果成功构建低表达Naa10的人食管鳞癌ECA109细胞株;3组转染后48 h及72 h Naa10表达及半数抑制浓度(IC50)差异均有统计学意义(P<0.05)。结论下调Naa10表达可增强人食管鳞癌ECA109细胞对DDP的敏感性。Objective To observe the effect of N-αacetyltransferase(Naa10p)expression on the sensitivity of cisplatin(DDP)to inhibit human esophageal squamous cell carcinoma ECA109 cells.Methods Human esophageal squamous cell carcinoma cell line ECA109 with low expression of Naa10 was constructed by small interfering RNA(siRNA).The experiment were divided into ECA109-siRNA-Naa10 group,ECA109-siRNA-NC group(transfection of invalid interference fragments),and the blank ECA109 cells were established as the ECA109-blank group.The interference efficiency was identified by fluorescence microscopy and Western blotting.CCk-8 method was used to detect the sensitivity of cells in each treatment group to DDP after drug treatment.Results A human esophageal squamous cell carcinoma ECA109 cell line with low Naa10p expression was successfully constructed.There were statistically significant differences in Naa10 expression and IC50 at 48 h and 72 h after transfection among the three groups(P<0.05).Conclusions Down-regulating the expression of Naa10p could enhance the sensitivity of human esophageal squamous cell carcinoma ECA109 cells to DDP.

关 键 词：N-α乙酰基转移酶 食管鳞癌 顺铂 敏感性 

分 类 号：R655.4[医药卫生—外科学]