A conservative distribution of tridomain NDP-heptose synthetases in actinobacteria  

作　　者：Yue Tang Wei Tang Min Wang Zhilong Zhang Yihua Chen 

机构地区：[1]State Key Laboratory of Microbial Resources and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China(Life Sciences)》2022年第5期1014-1023,共10页中国科学（生命科学英文版）

基　　金：supported in part by the National Key R&D Program of China(2020YFA0907700);the National Natural Science Foundation of China(32025002);the Center for Ocean Mega-Science,CAS(KEXUE2019GZ05)。

摘　　要：Heptoses are important structural components of Gram-negative bacterium cell wall and participate in bacterial colonization,infection,and immune recognition.Current knowledge of NDP-heptose originating from D-sedoheptulose 7-phosphate in Grampositive bacterium remains limited.Here,in silico analysis suggested that the special tridomain NDP-heptose synthetases with isomerase,kinase,and nucleotidyltransferase activities are conservatively distributed in Actinobacteria class of Gram-positive bacterium.Enzymatical characterization of the tridomain proteins from different strains showed that they are involved in ADPD-glycero-β-D-manno-heptose biosynthesis despite the unexpected discovery of kinase activities deficient in some proteins.The presence of three types of NDP-heptose synthetases in Gram-positive bacterium suggests that it is also a rich source of heptoses and the heptose moieties may play important roles in vivo.Our work updates the understanding of NDP-heptose biosynthesis in Gram-positive bacterium and lays a solid foundation for further physiological function explorations.

关 键 词：tridomain NDP-heptose synthetase NDP-heptose biosynthesis widespread distribution Gram-positive bacterium 

分 类 号：Q936[生物学—微生物学]