十味乳香丸对变应性接触性皮炎小鼠的抗炎止痒作用  

Anti-inflammatory and anti-pruritic effects of Shiwei Ruxiang Pills on mice with allergic contact dermatitis

作　　者：蒙凤萍 陈维武 李巧云 田娟 梅之南[1] MENG Feng-ping;CHEN Wei-wu;LI Qiao-yun;TIAN Juan;MEI Zhi-nan(College of Pharmacy,South-Central Minzu University,Wuhan 430074,China;Qizheng Tibetan Medicine Co.,Ltd,Lhasa 850000,China)

机构地区：[1]中南民族大学药学院,湖北武汉430074 [2]奇正藏药股份有限公司,西藏拉萨620100

出　　处：《中成药》2022年第5期1441-1447,共7页Chinese Traditional Patent Medicine

基　　金：国家科技重大专项重大新药创制项目(2017ZX09301060);国家民委领军人才项目(MZR20001)。

摘　　要：目的探讨十味乳香丸对变应性接触性皮炎(ACD)小鼠的抗炎止痒机制。方法采用方酸二丁酯诱导建立小鼠ACD模型,十味乳香丸灌胃给药,连续3 d。观察并记录小鼠的瘙痒行为学变化及右耳组织变化,测量右耳皮肤厚度差,HE染色及甲苯胺蓝染色检测小鼠表皮层厚度变化及真皮层肥大细胞数目,RT-qPCR法检测小鼠皮肤组织中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-4(IL-4)、Toll样受体8(TLR 8)的mRNA表达,ELISA法检测小鼠血清中TNF-α、IFN-γ、IL-1β、IL-6水平,Western blot法检测小鼠皮肤组织中MAPK信号通路蛋白表达。结果与模型组比较,十味乳香丸各剂量组小鼠挠痒次数减少(P<0.01),皮损及耳皮肤厚度不同程度减轻(P<0.01),表皮厚度降低、肥大细胞数目减少(P<0.01),皮肤和血清中相关炎症因子水平降低(P<0.01),皮肤组织p-ERK/ERK、p-P38/P38蛋白表达比值降低(P<0.05)。结论十味乳香丸对变应性接触性皮炎小鼠的抗炎止痒作用可能是通过调控Th1/Th2型免疫、抑制MAPK信号通路实现的。AIM To investigate the anti-inflammatory and anti-pruritic mechanism of Shiwei Ruxiang Pills on mice with allergic contact dermatitis(ACD).METHODS Upon mouse ACD models induced by dibutyl square acid,Shiwei Ruxiang Pills were administered intragastrically for three days in the trial group.Subsequently,the mice had their changes of pruritus behavior and right ear tissue observed and recorded;their right ear skin thickness measured;the changes of epidermal layer thickness and the number of mast cells in dermis by HE staining and toluidine blue staining;their mRNA expressions of TNF-α,IFN-γ,IL-1β,IL-6,IL-4 and TLR 8 in the skin tissue detected by RT-qPCR;their serum levels of TNF-α,IFN-γ,IL-1βand IL-6 investigated by ELISA;and their protein expressions of MAPK signaling pathway in skin tissues determined by Western blot.RESULTS Compared with the model group,groups intervened with Shiwei Ruxiang Pills shared overall decreases in scratching frequency(P<0.01),skin damage and ear thickness(P<0.01),epidermal thickness and mast cell number(P<0.01),levels of inflammatory factors in skin and serum(P<0.01),and expressions of p-ERK/ERK and p-P38/P38 protein ratios in skin tissue(P<0.05).CONCLUSION The anti-inflammatory and anti-pruritic effects of Shiwei Ruxiang Pills on ACD mice may be achieved by regulating Th1/Th2 type immunity and inhibiting MAPK signaling pathway.

关 键 词：十味乳香丸 变应性接触性皮炎(ACD) 抗炎 止痒 炎症因子 ERK/P38 

分 类 号：R285.5[医药卫生—中药学]