基于还原石墨烯和硫堇-金纳米粒子复合物的电化学DNA生物传感器的制备  

Fabrication of Electrochemical DNA Biosensor Based on Reduced Graphene and Thionin-gold Nanoparticle Composite

作　　者：董笑[1] 时安琪 耿明 DONG Xiao;SHI Anqi;GENG Ming(Department of Information Science,Huaibei Normal University,235000,Huaibei,Anhui,China;School of Chemistry and Materials Science,Anhui Normal University,241000,Wuhu,Anhui,China)

机构地区：[1]淮北师范大学信息学院,安徽淮北235000 [2]安徽师范大学化学与材料科学学院,安徽芜湖241000

出　　处：《淮北师范大学学报（自然科学版）》2022年第2期44-48,共5页Journal of Huaibei Normal University：Natural Sciences

基　　金：安徽省高校省级自然科学研究重点项目(KJ2019A0960,KJ2017A840)。

摘　　要：制备基于石墨烯和硫堇-金纳米复合物放大信号的三明治型电化学DNA生物传感器.石墨烯-金纳米粒子复合物作为传感基质有效地增大电极的比表面积和导电性,导致大量一端修饰巯基DNA探针固定在其表面.同时制备信使DNA-硫堇-金纳米粒子复合物,在目标DNA存在时,信使DNA-硫堇-金纳米粒子复合物通过杂交反应链接在电极表面形成三明治结构.采用差示脉冲伏安法记录硫堇产生电化学信号作为传感信号.在最佳实验条件下,目标DNA浓度为1.00×10^(-16) mol·L^(-1)~1.00×10^(-9) mol·L^(-1)范围内,硫堇峰电流大小与完全互补DNA浓度的对数呈良好的线性关系,其检测限达3.5×10^(−17) mol·L^(-1).An electrochemical sandwich DNA biosensor was fabricated based on graphene and gold nanoparticles-thionine composite(Au NPs-Thi)amplifying signal.A graphene-Au NPs composite increases the electrode surface area and conductivity,resulting in a mount of probe DNA with one end modified with sulfhydryl groups immobilized.Au NPs-thionine-rDNA composite was prepared.In presence of target DNA,an Au NPsthionine-rDNA composite was linked to the surface of electrode by hybridization to form sandwich structure.Different pulse voltmmetry was employed to measure the electrochemical signal of the thionin.Under the optimal experimental conditions,the peak current of thionine were linear with the logarithm of the target concentration from 1.00×10^(-16) mol·L^(-1) to 1.00×10^(-9) mol·L^(-1) with the detection limit of 3.5×10^(−17) mol·L^(-1).

关 键 词：石墨烯 金纳米粒子 硫堇 修饰电极 

分 类 号：O652.7[理学—分析化学]