荆芥提取物的抗氧化活性及成分研究  

The Research on Antioxidant Activity and Chemical Composition of Extraction of Schizonepeta Tenuifolia Brip

作　　者：张乐乐 高子强 汪菲菲 张盼盼 刘生杰 ZHANG Lele;GAO Ziqiang;WANG Feifei;ZHANG Panpan;LIU Shengjie(School of Life Science,Fuyan Normal University,236401,Fuyang,Anhui,China;College of Information Engineering,Fuyang Normal University,236401,Fuyang,Anhui,China)

机构地区：[1]阜阳师范大学生物与食品工程学院,安徽阜阳236041 [2]阜阳师范大学信息工程学院食品系,安徽阜阳236041

出　　处：《淮北师范大学学报（自然科学版）》2022年第2期49-55,共7页Journal of Huaibei Normal University：Natural Sciences

基　　金：安徽省教育厅自然科学重点项目(KJ2019A0951);阜阳师范大学信息工程学院青年人才重点项目(2021XGRCXM004);阜阳师范大学横向项目(SXHZ202009);阜阳地方农产品食品工艺开发科研创新团队(FX2020KCT02)。

摘　　要：为探究荆芥水相提取物和有机相提取物的体外抗氧化活性.采用2,2-二苯基-1-苦基肼(DPPH法)、2,2′-二氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS法)、亚铁离子与三吡啶基三嗪(FRAP法)对其进行评价.实验结果表明:荆芥水相提取物和有机相提取物都具有抗氧化活性,其中水相提取物抗氧化能力显著大于有机相提取物的抗氧化能力(p<0.01).3种体系中,ABTS体系中抗氧化性活性最高,每克荆芥水相提取物的抗氧化活性相当于4.95±0.06 mmol的水溶性维生素E(Trolox).通过气相质谱分析荆芥水相和有机相成分,主要由油酸甲酯和亚油酸甲酯组成.In order to explore the antioxidant activity of extraction of Schizonepeta tenuifolia Brip,this essay uses 2,2-diphenyl-1-picrylhydrazyl(DPPH)method,Diammonium 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate)(ABTS)method,ferric reduction ability plasmaassay(FRAP)method to compare the antioxidant activity between water extracts and n-hexaneextracts.The results show that both water and n-hexaneextracts from Schizonepeta tenuifolia Brip had antioxidant activity.The antioxidant activity of water extracts was significantly higher than that of the n-hexaneextracts(p<0.01),and ABTS radical activity was the highest among the three methods,4.95±0.06 mmol Trolox per sample.The main components were 9,12-Octadecadienoic(Z,Z)-methyl esterand 9-Octadecenoic acidmethyl ester by Gas chromatography-mass spectrometry.

关 键 词：荆芥提取物 DPPH ABTS FRAP 

分 类 号：Q946[生物学—植物学]