A major and stable QTL for wheat spikelet number per spike validated in different genetic backgrounds  

作　　者：DING Pu-yang MO Zi-qiang TANG Hua-ping MU Yang DENG Mei JIANG Qian-tao LIU Ya-xi CHEN Guang-deng CHEN Guo-yue WANG Ji-rui LI Wei QI Peng-fei JIANG Yun-feng KANG Hou-yang YAN Gui-jun WEI Yu-ming ZHENG You-liang LAN Xiu-jin MA Jian 

机构地区：[1]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Ministry of Science and Technology/Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,P.R.China [2]College of Resources,Sichuan Agricultural University,Chengdu 611130,P.R.China [3]College of Agronomy,Sichuan Agricultural University,Chengdu 611130,P.R.China [4]UWA School of Agriculture and Environment and The UWA Institute of Agriculture,Faculty of Science,University of Western Australia,Crawley 6009,Australia

出　　处：《Journal of Integrative Agriculture》2022年第6期1551-1562,共12页农业科学学报（英文版）

基　　金：supported by the projects from the Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China (2020YJ0140 and 2021YJ0503);the International Science and Technology Cooperation and Exchanges Program of Science and Technology Department of Sichuan Province, China (2021YFH0083 and 2022YFH0053);the National Natural Science Foundation of China (31971937 and 31970243);the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province, China

摘　　要：The spikelet number per spike(SNS)contributes greatly to grain yield in wheat.Identifying various genes that control wheat SNS is vital for yield improvement.This study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci(QTLs)for SNS.One of them(QSns.sau-2SY-2D.1)was reported previously,while the other(QSns.sau-2SY-7A)was newly detected and further analyzed in this study.QSns.sau-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21-40.78%of the phenotypic variances.QSns.sau-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm 7AL.The contributions and interactions of both major QTLs were further analyzed and discussed.The effect of QSns.sau-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F_(2:3) population and a panel of 101 high-generation breeding wheat lines.Furthermore,several genes including the previously reported WHEAT ORTHOLOG OF APO1(WAPO1),an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1(APO1)related to SNS,were predicted in the interval of QSns.sau-2SY-7A.In summary,these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major QTLs.

关 键 词：yield potential QTL detection QTL validation predicted genes tightly linked KASP marker 

分 类 号：S512.1[农业科学—作物学]