Genome-wide identification,evolutionary selection,and genetic variation of DNA methylation-related genes in Brassica rapa and Brassica oleracea  

作　　者：AN Feng ZHANG Kang ZHANG Ling-kui LI Xing CHEN Shu-min WANG Hua-sen CHENG Feng 

机构地区：[1]Collaborative Innovation Center for Efficient and Green Production of Agriculture in Zhejiang Mountainous Areas,College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300,P.R.China [2]Institute of Vegetables and Flowers,Chinese Academy of Agricul tural Sciences,Beijing 100081,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第6期1620-1632,共13页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (NSFC;31872105 and 31801862);the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences, and the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs, China

摘　　要：DNA methylation plays an important role in plant growth and development,and in regulating the activity of transposable elements(TEs).Research on DNA methylation-related(DMR)genes has been reported in Arabidopsis,but little research on DMR genes has been reported in Brassica rapa and Brassica oleracea,the genomes of which exhibit significant differences in TE content.In this study,we identified 78 and 77 DMR genes in Brassica rapa and Brassica oleracea,respectively.Detailed analysis revealed that the numbers of DMR genes in different DMR pathways varied in B.rapa and B.oleracea.The evolutionary selection pressure of DMR genes in B.rapa and B.oleracea was compared,and the DMR genes showed differential evolution between these two species.The nucleotide diversity(π)and selective sweep(Tajima’s D)revealed footprints of selection in the B.rapa and B.oleracea populations.Transcriptome analysis showed that most DMR genes exhibited similar expression characteristics in B.rapa and B.oleracea.This study dissects the evolutionary differences and genetic variations of the DMR genes in B.rapa and B.oleracea,and will provide valuable resources for future research on the divergent evolution of DNA methylation between B.rapa and B.oleracea.

关 键 词：DNA methylation Brassica rapa Brassica oleracea evolutionary selection genetic variation gene expression 

分 类 号：Q943[生物学—植物学]