高通量测序技术在害虫和害螨抗药性研究中的应用  

Application of high-throughput sequencing in the study of acaricide resistance

作　　者：杨静 张愉英 高越 刘中芳 张鹏九 史高川 周序国 范仁俊[1] Yang Jing;Zhang Yuying;Gao Yue;Liu Zhongfang;Zhang Pengjiu;Shi Gaochuan;Zhou Xuguo;Fan Renjun(Shanxi Key Laboratory of Integrated Pest Management in Agriculture,College of Plant Protection,Shanxi Agricultural University,Taiyuan 030031,Shanxi Province,China;College of Plant Protection,HunanAgricultural University,Changsha 410125,Hunan Province,China;Pomology Institute,Shanxi Agricultural University,Taigu 030815,Shanxi Province,China;Department of Entomology,University of Kentucky,Lexington 40546,Kentucky,USA)

机构地区：[1]山西农业大学植物保护学院,农业有害生物综合治理山西省重点实验室,太原030031 [2]湖南农业大学植物保护学院,长沙410125 [3]山西农业大学果树研究所,太谷030815 [4]肯塔基大学昆虫系,美国肯塔基州40546

出　　处：《植物保护学报》2022年第1期383-388,共6页Journal of Plant Protection

基　　金：国家自然科学基金(32102224);山西省回国留学人员科研资助项目(2021-073)。

摘　　要：螨类是陆生动物中仅次于昆虫的第2大类群。长期以来,对害螨抗药性的研究都落后于害虫。近年来,随着高通量测序技术的出现,开辟了害虫和害螨抗性机理研究和抗性监测的新局面。本文概述了高通量测序技术应用于害虫和害螨抗药性机理研究已取得的进展,介绍了利用高通量测序技术开发的抗药性监测新方法,并提出了害虫和害螨抗药性机理研究和田间种群抗性水平监测的发展方向。Mites are the second-largest group of terrestrial animals following insects.The research in acaricide resistance of mites,however,has been substantially lagged behind.In recent years,the advent of genomics era has afforded researchers with high-throughput sequencing technologies to address outstanding biological questions.In this review,we summarized the current progresses in mechanistic studies in acaricide resistance of pest insects and mites and resistance monitoring using high-throughput sequencing approaches.Finally,we concluded this review by sharing our perspectives on future research avenues.

关 键 词：高通量测序技术 抗药性机理 抗药性监测 害虫 害螨 

分 类 号：S433[农业科学—农业昆虫与害虫防治]