动物细胞低氧培养相关调控因子研究进展  

Research Progress on Regulatory Factors Related to Hypoxic Culture of Animal Cells

作　　者：张兰 姚一凡 陈树吾 马芳芳 许瑾 杨琨 ZHANG Lan;YAO Yifan;CHEN Shuwu;MA Fangfang;XU Jin;YANG Kun(College of Life Sciences and Engineering,Northwest Minzu University,Lanzhou 730030,China;Gansu Animal Cell Technology Innovation Center,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China;Key Laboratory of Bioengineering and Technology,State Ethnic Affairs Commission,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学生命科学与工程学院,兰州730030 [2]西北民族大学生物医学研究中心,甘肃省动物细胞技术创新中心,兰州730030 [3]西北民族大学生物医学研究中心,生物工程与技术国家民委重点实验室,兰州730030

出　　处：《中国畜牧兽医》2022年第5期1926-1933,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31860687);甘肃省自然科学基金(21JR11RA024);中央高校创新团队项目(31920200004);教育部动物医学生物工程创新团队(IRT-17R88)。

摘　　要：近年来低氧细胞培养逐渐成为研究热点,低氧条件下可提高细胞体外扩增的生长动力学、细胞的增殖率及干细胞分化等功能。缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)是应答低氧应激的关键因子,也是介导低氧信号的转导中枢,与其相关的血管内皮生长因(vascular endothelia growthfactor,VEGF)、低氧促有丝分裂因子(hypoxia-induced mitogenic factor,HIMF)及促红细胞生成素(erythropoietin,EPO)等是其重要的靶基因,它们之间的相互作用对低氧条件下细胞的发生、发展有重要作用。笔者对低氧培养细胞的研究现状及低氧相关细胞因子的调控机制及作用进行概述,以期为探索细胞低氧机理提供理论依据,为低氧适应性及高原疾病治疗提供更多的研究思路。In recent years,hypoxic cell culture has gradually become a research hotspot.It has been reported that hypoxic conditions can improve cell expansion in vitro growth kinetics,cell proliferation rate and stem cell differentiation and other functions.Hypoxia inducible factor-1α(HIF-1α)is a key factor in response to hypoxia stress and also a transduction center mediating hypoxia signal.Vascular endothelia growth factor(VEGF),hypoxia-induced mitogenic factor(HIMF)and erythropoietin(EPO)and so on are important target genes and their interactions play an important role in cell development under hypoxia conditions.In this paper,the research status of hypoxic cultured cells and the regulation mechanism and role of hypoxia-related factors were summarized,so as to provide theoretical data for exploring the mechanism of cell hypoxia,and provide more research ideas for the adaptation to altitude hypoxia and the treatment of altitude diseases.

关 键 词：低氧培养细胞 低氧相关因子 调控机制 

分 类 号：S852.33[农业科学—基础兽医学]