新型滴鼻灭活鹦鹉热衣原体疫苗的免疫效力评价  

Immune Efficacy of Novel Nasal Vaccine of Inactivated EB Against Chlamydia psittaci Challenge

作　　者：陈思宇 隋卓君 王占新 李强[1] 陈艺 王飞 陈爽 刘心怡 严专强 何诚[1] CHEN Siyu;SUI Zhuojun;WANG Zhanxin;LI Qiang;CHEN Yi;WANG Fei;CHEN Shuang;LIU Xinyi;YAN Zhuanqiang;HE Cheng(China-Pakistan Agricultural Science and Technology and Agricultural Service Center,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;Wen’s Food Group Co.,Ltd.,Yunfu 527499,China)

机构地区：[1]中国农业大学动物医学院,中巴农业科技创新与服务中心,北京100193 [2]温氏食品集团股份有限公司,云浮527499

出　　处：《中国畜牧兽医》2022年第5期1951-1959,共9页China Animal Husbandry & Veterinary Medicine

基　　金：科技部专项(2016YFE0127100);温氏科研基金项目(202005410510320);国家级大学生创业训练计划项目(202110019092x)。

摘　　要：【目的】探究由霍乱弧菌菌影(Vibrio cholerae ghosts,VCG)和纳米壳聚糖凝胶(Gel)组合制作的一种新型灭活衣原体疫苗复合佐剂是否可增强鹦鹉热衣原体原体(elementary body,EB)灭活抗原诱导动物机体产生的免疫应答。【方法】将60只7日龄SPF鸡随机分为4个组,分别为:EB+VCG+Gel组、EB+VCG组、Gel组和EB组。通过滴鼻途径免疫鸡群,免疫2次,每次间隔14 d,免疫后检测鸡血清中IgG抗体水平、淋巴细胞增殖指数、CD4^(+)/CD8^(+)T细胞比例、细胞因子(白介素-4(IL-4)、IL-10、IL-12和干扰素-γ(IFN-γ))含量及攻毒后鸡喉头排菌量和肺脏的病理损伤程度。【结果】与Gel和EB组相比,EB+VCG+Gel和EB+VCG组IgG抗体水平、淋巴细胞增殖指数、IFN-γ含量和CD4^(+)/CD8^(+)T细胞比值显著或极显著升高(P<0.05;P<0.01)。此外,与Gel和EB组相比,攻毒后第12天,EB+VCG+Gel组喉头排菌量极显著降低(P<0.01),攻毒后第7天,肺脏损伤评分极显著降低(P<0.01)。【结论】壳聚糖凝胶、VCG、灭活衣原体EB组合构成的新型滴鼻衣原体疫苗经鼻腔接种动物后可刺激动物机体产生良好的体液免疫和细胞免疫应答,阻断鹦鹉热衣原体经呼吸道途径传播,防止鹦鹉热衣原体从动物向人群传播。【Objective】The purpose of this study was to investigate the effects of Vibrio cholerae ghosts(VCG)and chitosan gel(Gel)as compound adjuvants on enhancement of immunity of inactivated elementary body(EB)against Chlamydia psittaci infection.【Method】Sixty SPF chickens aged 7 days were randomly divided into 4 groups,15 birds per group,including EB+VCG+Gel group,EB+VCG group,Gel group and EB group.SPF chickens were immunized twice via nasal drop at an interval of 14 days.Specific IgG antibody levels,lymphocyte proliferation index,proportion of CD4^(+)/CD8^(+)T cells,contents of cytokines(IL-4,IL-10,IL-12 and IFN-γ)were determined post immunization.Afterwards,throat bacteria excretion and lung lesions were detected post challenge with virulent Chlamydia psittaci strain.【Result】Compared with Gel and EB groups,IgG antibody level,lymphocyte proliferation index and IFN-γcontent and the ratio of CD4^(+)/CD8^(+)T cells in EB+VCG+Gel and EB+VCG groups were significantly or extremely significantly increased(P<0.05;P<0.01).In addition,compared with Gel and EB groups,on the 12th day after challenge,the amount of laryngeal bacteria excretion in EB+VCG+Gel group was extremely significantly lower(P<0.01),on the 7th day after challenge,the lung lesion was extremely significantly lower(P<0.01).【Conclusion】The novel nasal vaccine containing chitosan gel,VCG and inactivated EB was a promising approach by enhancing humoral and cellular responses,blocking transition via respiratory tracts,contributing to eradication the transmission via infected animals to human beings.

关 键 词：鹦鹉热衣原体 滴鼻疫苗 免疫效力 霍乱弧菌菌影(VCG) 壳聚糖凝胶 鸡 

分 类 号：S852.67[农业科学—基础兽医学]