绿原酸通过增强RsmA的表达抑制铜绿假单胞菌生物被膜的形成  

Chlorogenic acid represses biofilm formation of Pseudomonas aeruginosa via increasing RsmA expression

作　　者：唐小丽 韦俊彬[2] 封毅 乐宁 梁丹娜 卓少元 尚立国 TANG Xiaoli;WEI Junbin;FENG Yi;LE Ning;LIANG Danna;ZHUO Shaoyuan;ShANG Liguo(Faculty of Nursing,Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;Teaching and Experimental Center,Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;School of Basic Medical Science,Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;Faculty of Chinese Medicine Science,Guangxi University of Chinese Medicine,Nanning 530222,Guangxi,China)

机构地区：[1]广西中医药大学护理学院,广西南宁530200 [2]广西中医药大学教学与实验中心,广西南宁530200 [3]广西中医药大学基础医学院,广西南宁530200 [4]广西中医药大学赛恩斯新医药学院,广西南宁530222

出　　处：《微生物与感染》2021年第5期322-330,共9页Journal of Microbes and Infections

基　　金：广西自然科学基金青年基金(2019GXNSFBA245078);广西中医药大学2018年引进博士科研启动基金(2018BS008);广西中医药大学校级科研项目(2019QN008)。

摘　　要：铜绿假单胞菌是常见的人类条件致病菌,其生物被膜的形成会增强菌体的耐药性。已有文献报道绿原酸可抑制铜绿假单胞菌生物被膜的形成,本研究在此基础上主要探究了其对全局性次级代谢调控系统Gac-Rsm表达的影响。结果显示,绿原酸可抑制铜绿假单胞菌生物被膜形成的能力,降低胞外总多糖合成量,但关键胞外多糖psl的合成酶基因pslA转录未受影响,还可增强Gac-Rsm系统中关键调控因子RsmA的表达水平,降低细胞内关键信使分子环二鸟苷酸(cyclic dimeric guanosine monophosphate,c-di-GMP)水平。结果表明,绿原酸可通过增强RsmA的表达来抑制铜绿假单胞菌生物被膜的形成。Pseudomonas aeruginosa(P.aeruginosa)is a common human opportunistic pathogen,and the biofilm formation could enhance its antibiotic tolerance.Chlorogenic acid(CA)has been reported to repress the biofilm formation of P.aeruginosa,so the present study aims to further explore the regulatory mechanism of CA,especially the effect of CA on the expression of Gac-Rsm(global secondary metabolism regulation system).The results showed that CA could repress the biofilm formation of P.aeruginosa,reduce exopolysaccharide production,although the transcription level of pslA(synthetase gene of key exopolysaccharide psl)was not significantly changed.The expression of RsmA(core regulator in Gac-Rsm system)was enhanced and the level of key signal molecule cyclic dimeric guanosine monophosphate(c-di-GMP)was reduced.It is suggested that CA represses the biofilm formation of P.aeruginosa via increasing the expression of RsmA.

关 键 词：铜绿假单胞菌 生物被膜 绿原酸 RSMA 环二鸟苷酸 

分 类 号：R378[医药卫生—病原生物学]