黄瓜绿斑驳花叶病毒的遗传多样性与检测技术研究进展  

Research progresses on genetic diversity and detection technology of cucumber green mottle mosaic virus

作　　者：罗金燕[1] 吴志峰 谢婷[2] 陈磊[1] 杜伟 李斌[2] Luo Jinyan;Wu Zhifeng;Xie Ting;Chen Lei;Du Wei;Li Bin(Shanghai Agriculture Technology Extension&Service Center,Shanghai 201103,China;Institute of Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang Province,China;Agricultural Technology Extension Station of Ningxia Hui Autonomous Region,Yinchuan 750001,Ningxia Hui Autonomous Region,China)

机构地区：[1]上海市农业技术推广服务中心,上海201103 [2]浙江大学生物技术研究所,杭州310058 [3]宁夏回族自治区农业技术推广总站,银川750001

出　　处：《植物保护学报》2022年第2期460-470,共11页Journal of Plant Protection

基　　金：上海市科技兴农项目(2019-02-08-00-08-F01150);宁夏回族自治区重点研发计划(2020BBF03004)。

摘　　要：黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)是我国重要的植物检疫性有害生物,预防其扩散和传播的关键在于准确、灵敏和快速的检测技术。该文总结分析NCBI数据库中已测定CGMMV分离物的基因组多样性;比较目前CGMMV主要检测技术的优缺点;归纳总结当前广泛用于检测CGMMV的逆转录-聚合酶链反应(reverse transcription PCR,RT-PCR)引物,并针对RT-PCR技术的最新研究进展和存在的一些不足进行讨论;最后对CGMMV检测技术的未来发展方向进行展望。Cucumber green mottle mosaic virus(CGMMV) is an important plant quarantine pest in China. The key to prevent its spread lies in accurate, sensitive and rapid detection technology. This paper firstly analyzed and summarized the diversity of the sequenced CGMMV genomes available in the NCBI database;compared the advantages and disadvantages of the current main CGMMV detection technologies;summed up the CGMMV detection primers that widely used in reverse transcription PCR(RTPCR), and then discussed the latest research progresses and some shortcomings of RT-PCR technology.Finally, this paper looked forward to the future direction of CGMMV detection technology.

关 键 词：黄瓜绿斑驳花叶病毒 检疫 基因组 遗传多样性 检测技术 RT-PCR 

分 类 号：S432.41[农业科学—植物病理学]