黄秋葵果实老化与内切葡聚糖酶基因的关系分析  

Studies on the Relationship between Fruit Aging Mechanism and Endoglucanase Gene Family of Hibiscus esculentus L.

作　　者：李永平[1] 陈朝文 马慧斐 康玉妹[1] 白昌辉 薛珠政[1] 温庆放[1] LI Yongping;CHEN Chaowen;MA Huifei;KANG Yumei;BAI Changhui;XUE Zhuzheng;WEN Qingfan(Fujian Key Laboratory of Vegetable Genetics and Breeding,Crops Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Quanzhou Agricultural Research Institute,Quanzhou 362212,China)

机构地区：[1]福建省蔬菜遗传育种重点实验室/福建省农业科学院作物研究所,福州350013 [2]泉州市农业科学研究所,泉州362212

出　　处：《中国细胞生物学学报》2022年第2期257-267,共11页Chinese Journal of Cell Biology

基　　金：福建省属公益类科研院所基本科研专项(批准号:2019R1026-9);国家大宗蔬菜产业技术体系福州综合试验站项目(批准号:CARS-23-G-53);福建省农科院科技创新平台专项(批准号:CXPT202001)资助的课题。

摘　　要：黄秋葵(Hibiscus esculentus L.)果实极易老化,采收期和货架期都极短,为研究黄秋葵老化的机理,该文通过实验测定了黄秋葵果实发育过程中和采后纤维素含量变化,显微镜观察了细胞纤维素组织结构变化。黄秋葵果实老化过程中纤维素含量大量增加,细胞内纤维组织增多,推测纤维素增加是黄秋葵果实老化的主导因素。植物内切葡聚糖酶基因与纤维素的合成密切相关,该研究从黄秋葵果实转录组测序的RNA-seq数据库中筛选得到7条功能注释为endoglucanase基因家族的片段序列,克隆获得黄秋葵内切葡聚糖酶基因家族的endoglucanase1、endoglucanase3、endoglucanase6、endoglucanase10、endoglucanase11、endoglucanase23、endoglucanas25基因全长,分别将其命名为HeCEL1、HeCEL3、HeCEL6、HeCEL10、HeCEL11、HeCEL23、HeCEL25 7个基因,它们均属于GH9家族。通过荧光定量PCR分析黄秋葵内切葡聚糖酶基因家族7个基因的表达情况,结果表明黄秋葵果实发育时HeCEL3、HeCEL10、HeCEL25表达模式相同,HeCEL6、HeCEL23表达模式一致;HeCEL25果实采后0~2天表达量平稳,其余6个基因在采后1天表达水平急剧下降。黄秋葵果实发育过程中的纤维素、采后常温贮藏其纤维素含量与HeCEL3、HeCEL6、HeCEL10、HeCEL25、HeCEL23表达量呈显著负相关,推测HeCEL3、HeCEL6、HeCEL10、HeCEL25、HeCEL23在黄秋葵果实纤维素积累中起重要作用,与黄秋葵果实的老化密切相关。Hibiscus esculentus L.is very easy to age,and its harvest time and shelf life are very short.In order to study the aging mechanism of Hibiscus esculentus L.fruit,the changes of cellulose content as well as the cell cellulose structure were analysed during the fruit development and the postharvest period.It was found that in the aging process of Hibiscus esculentus L.fruit,both the content of cellulose and the cell cellulose tissue were increased.It was suggested that the increase of cellulose was the leading factor of the Hibiscus esculentus L.fruit aging.Endoglucanase genes in plants are closely related to cellulose synthesis.In this study,seven fragments which were functionally annotated as endoglucanase gene family were screened from the RNA-seq database of Hibiscus esculentus L.fruit transcriptome sequencing,and 7 full-length genes of endoglucanase gene family,endoglucanase1,endoglucanase3,endoglucanase6,endoglucanase10,endoglucanase11,endoglucanase23 and endoglucanas25 were cloned and named as HeCEL1,HeCEL3,HeCEL6,HeCEL10,HeCEL11,HeCEL23 and HeCEL25 respectively.All these genes belong to the GH9 family.Expression patterns of the these genes were carried out by qRT-PCR (reverse transcript qunntitative PCR).It showed that expression patterns of HeCEL3,HeCEL10 and HeCEL25 were in the same,and HECEL6 was in concordance with HeCEL23,too.The expression of HeCEL25 was stable at 0-2 days after harvest,while the expression of the other 6 genes decreased sharply at 1 day after harvest.During the development of Hibiscus esculentus L.fruit,the content of cellulose stored at room temperature after harvest was significantly negatively correlated with the expression of HeCEL3,HeCEL10,HeCEL25,HeCEL6 and HeCEL23,and it was speculated that HeCEL3,HeCEL10,HeCEL25,HeCEL6 and HeCEL23 played an important role in cellulose accumulation in Hibiscus esculentus L. fruit,which was closely related to the aging of Hibiscus esculentus L. fruit.

关 键 词：黄秋葵 老化 纤维素 内切葡聚糖酶 

分 类 号：S649[农业科学—蔬菜学]