麻疯树叶中3个新的糖苷类化合物  

Three new glycosides from leaves of Jatropha curcas

作　　者：张伟[1] 罗锐 孙静[1] 张东博[1] ZHANG Wei;LUO Rui;SUN Jing;ZHANG Dong-bo(State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi&Education Ministry,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学陕西中药资源产业化省部共建协同创新中心秦药特色资源研究开发国家重点实验室(培育),陕西咸阳712046

出　　处：《中草药》2022年第9期2597-2604,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81703392);陕西高校青年创新团队。

摘　　要：目的 研究麻疯树Jatrophacurcas叶的化学成分及其抗炎活性。方法 采用硅胶、大孔吸附树脂、半制备HPLC等色谱技术进行分离纯化,通过理化性质和NMR、HR-ESI-MS、ECD等现代波谱学技术对化合物的结构进行鉴定;采用Griess法测定化合物对脂多糖(LPS)诱导小鼠巨噬细胞(RAW264.7)释放炎症介质一氧化氮的抑制作用。结果 从麻疯树叶的正丁醇部位中分离得到6个化合物,分别鉴定为(+)-jatrointelignanB-4′′-O-β-D-glucopyranoside(1)、3-hydroxy-4,7-megastigmadien-3-one 9-O-[α-arabinopyranosyl-(1→6)-β-glucopyranoside](2)、β-紫罗兰酮-4-O-[β-呋喃芹糖-(1→6)-β-吡喃葡萄糖苷](3)、byzantionosideB(4)、foliasalaciosideB1(5)、(6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one9-O-[α-Larabinopyranosyl-(1→6)-β-D-glucopyranoside](6)。结论 化合物1~3为新化合物,命名为盐边麻疯苷A~C。化合物4~6为首次从该属植物中分离得到。所有化合物在50.0μmol/L浓度时,对一氧化氮的产生均无抑制作用。Objective To study the chemical compositions from leaves of Jatropha curcas and their anti-inflammatory activities.Methods The compounds were isolated and purified by silica gel,HP-20 macroporous resin and semi-preparative HPLC,and their structures were elucidated by physical and spectroscopic analysis.All compounds were tested for inhibitory activities against LPS-induced nitric oxide production in RAW264.7 cells by Griess method.Results Three new compounds,(+)-jatrointelignan B-4′′-O-β-D-glucopyranoside(1),3-hydroxy-4,7-megastigmadien-3-one 9-O-[α-arabinopyranosyl-(1→6)-β-glucopyranoside](2),β-ionone-4-O-[β-apiofuranosyl-(1→6)]-β-glucopyranoside(3),and three known ones,byzantionoside B(4),foliasalacioside B1(5),and(6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one 9-O-[α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside](6),were identified from n-Bu OH extract of J.curcas leaves.Conclusion Compounds 1—3 are new compounds named jatrcaside A-C.Compounds 4—6 are isolated from the Jatropha genus for the first time.All compounds show no inhibition against LPS-induced NO production in a macrophage cell line RAW264.7 at 50.0 μmol/L.

关 键 词：大戟科 麻疯树 盐边麻疯苷A 盐边麻疯苷B 盐边麻疯苷C 抗炎活性 byzantionoside B foliasalacioside B_(1) 

分 类 号：R284.1[医药卫生—中药学]