艾草种子的化学成分研究  

Chemical constituents from seeds of Artemisia argyi

作　　者：薛贵民 赵晨光 薛金凤 星贵福 赵珍珠 杜锟 司盈盈 孙彦君[1,2] 冯卫生 XUE Gui-min;ZHAO Chen-guang;XUE Jin-feng;XING Gui-fu;ZHAO Zhen-zhu;DU Kun;SI Ying-ying;SUN Yan-jun;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术研究中心,河南郑州450046

出　　处：《中草药》2022年第9期2605-2611,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然基金资助项目(82003606);河南省高等学校重点科研项目(21A360002);河南省科技攻关项目(182102310288);河南中医药大学科研苗圃工程项目(MP2020-29);河南省高等学校大学生创新训练计划项目(CXXM[2019]0064)。

摘　　要：目的研究艾草Artemisiaargyi种子部位的化学成分。方法利用现代色谱分离技术包括大孔树脂、C18-ODS、Sephadex LH-20及制备型HPLC进行分离纯化,根据NMR、MS等波谱数据及化合物的理化性质鉴定化合物结构。结果从艾叶种子95%乙醇提取物中分离得到18个化合物,分别鉴定为desacetylmatricarin(1)、3-methoxytanapartholide(2)、3α-chloro-8α-acetoxy-4β,10α-dihydroxy-1β,2β-epoxy-5α,7αH-guai-11(13)-en-12,6α-olide(3)、1α,6α,8α-trihydroxy-5α,7βH-guaia-3,10(14),11(13)-trien-12-oic acid(4)、11α,13-二氢魃蒿内酯(5)、魃蒿内酯(6)、tuberiferin(7)、8-acetylrupicolin B(8)、lirioresinol A(9)、丁香树脂酚(10)、dehydroleucodine(11)、leukodin(12)、matricarin(13)、8-acetylrupicolin A(14)、methyl-4S-6α-hydroxy-3-oxoeudesma-1,11(13)-dien-12-oate(15)、滨蒿内酯(16)、异东莨菪素(17)、8-羟基-6,7-二甲氧基香豆素(18)。结论化合物4为首次从该属植物中分离得到,化合物2、3、5、8~12、14~16、18首次从艾草中分离得到。化合物6~8、11、15在10μmol/L时对胃癌细胞MGC-803抑制率为61.8%~67.5%,具有显著的抑制活性。Objective To study the chemical constituents from the seeds of Artemisia argyi.Methods The chemical constituents from the seeds of A.argyi were isolated and purified by modern chromatographic separation techniques such as macroporous resin,C18-ODS,Sephadex LH-20 and preparative HPLC.The structures were identified by the NMR,MS spectra data and physicochemical properties analysis.Results Eighteen compounds were isolated and identified from 95% ethanol extract from the seeds of A.argyi,and their structures were identified as desacetylmatricarin(1),3-methoxytanapartholide(2),3α-chloro-8α-acetoxy-4β,10α-dihydroxy-1β,2β-epoxy-5α,7αH-guai-11(13)-en-12,6α-olide(3),1α,6α,8α-trihydroxy-5α,7βH-guaia-3,10(14),11(13)-trien-12-oic acid(4),11α,13-dihydroyomogin(5),yomogin(6),tuberiferin(7),8-acetylrupicolin B(8),lirioresinol A(9),syringaresinol(10),dehydroleucodine(11),leukodin(12),matricarin(13),8-acetylrupicolin A(14),methyl-4 S-6α-hydroxy-3-oxoeudesma-1,11(13)-dien-12-oate(15),scoparone(16),isoscopoletin(17),and 8-hydroxy-6,7-dimethoxycoumarin(18).Conclusion Compound 4 is separated from the genus of Artemisia for the first time.Compounds 2,3,5,8-12,14-16 and 18 are isolated from A.argyi for the first time.Cytotoxic effects of compounds 1-18 are investigated in four different gastric cancer cell lines,and the results show that compounds 6-8,11 and 15 have significant inhibitory activity on MGC-803 cell line at 10 μmol/L with the inhibition rate in the range 61.8%-67.5%.

关 键 词：艾草 倍半萜 木脂素 香豆素 滨蒿内酯 8-羟基-6 7-二甲氧基香豆素 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]