HPLC测定蒙药沙日·毛都-9丸中羟基红花黄色素A的含量  

Determination of hydroxysafflor yellow A in Mongolian medicine Shari·Maodu-9 pills by HPLC

作　　者：赵虎义 张德瑞 李彦铮 吕颖 ZHAO Hu-yi;ZHANG De-rui;LI Yan-zheng;LV Ying(Chifeng Products Quality Inspection And Testing Center,Inner Mongolia Autonomous Region,Chifeng 024000,China)

机构地区：[1]赤峰市产品质量检验检测中心,内蒙古赤峰024000

出　　处：《中国民族医药杂志》2022年第5期59-60,共2页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古蒙中药标准建设项目(编号:JYCZ18-027-6)。

摘　　要：目的:建立HPLC测定蒙药沙日·毛都-9丸中羟基红花黄色素A的含量。方法:色谱柱采用Diamonsil Plus(250 mm×4.6 mm,5μm),流动相:甲醇-乙腈-0.7%磷酸溶液(用三乙胺调节pH至6.0±0.1)(26∶2∶72);流速:1.0 mL/min;检测波长:403 nm;柱温:30℃。结果:羟基红花黄色素A在7.979~79.79μg/mL范围内与峰面积线性关系良好,回归方程为Y=3.269×10^(4)X+6.177×10^(3)(R^(2)=0.99997),平均加样回收率为98.1%,RSD为0.58%(n=9)。结论:该方法科学、简便、专属性较强,可有效控制沙日·毛都-9丸中羟基红花黄色素A的质量。Objective:To establish a HPLC method for the determination of hydroxysafflor yellow A in Mongolian medicine Shari·Maodu-9 pills.Method:Diamonsil Plus(250 mm×4.6 mm,5μm)was used for the chromatographic column,mobile phase:methanol,acetonitrile,and 0.7%phosphoric acid solution(Adjusted with triethylamine to pH 6.0±0.1)(26∶2∶72);Flow rate:1.0 m L/min;detection wavelength:403 nm;column temperature:30℃.Results:Hydroxysafflor yellow A had a good linear relationship with the peak area in the range of 7.979~79.79μg/mL.The regression equation was Y=3.269±10^(4)X+6.177±10^(3)(R^(2)=0.99997).The average sample recovery was 98.1%,RSD is 0.58%(n=9).Conclusion:The method is scientific,simple and specific,and can effectively control the quality of hydroxysafflor yellow A in Shari·Maodu-9 pills.

关 键 词：沙日·毛都-9丸 羟基红花黄色素A 高效液相色谱法 含量测定 

分 类 号：R291.2[医药卫生—民族医学]