重组人生长激素对局灶性脑缺血再灌注小鼠运动功能的改善作用及其机制  

Improvement effect of recombinant human growth hormone on motor function in mice with focal cerebral ischemia-reperfusion and its mechanism

作　　者：林韬 李莹华 连雅雯 陈晓伟[2] 万芪 LIN Tao;LI Yinghua;LIAN Yawen;CHEN Xiaowei;WAN Qi(Department of Neurosurgery,Affiliated Hospital,Qingdao University,Institute of Neuroregeneration&Neurorehabilitation,Qingdao University,Qingdao 266071,China;Department of Rehabilitation Medicine,First Hospital,Jilin University,Changchun 130021,China)

机构地区：[1]青岛大学附属医院神经外科青岛大学神经再生与康复研究院,山东青岛266071 [2]吉林大学第一医院康复医学科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2022年第3期568-574,共7页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金项目(82071385)。

摘　　要：目的:观察重组人生长激素对局灶性脑缺血再灌注小鼠运动功能的影响,探讨其可能的作用机制。方法:选取C57BL/6J小鼠24只,采用改良Zea-Longa线栓法建立脑缺血再灌注损伤模型,随机分为生长激素治疗组(脑缺血再灌注+生长激素,SG组)和对照组(脑缺血再灌注+生理盐水,SS组),每组12只。SG组小鼠于造模后48 h开始颈部皮下注射重组人生长激素,剂量为1.4 mg·kg^(-1)·d^(-1),持续14 d;SS组小鼠注射生理盐水,注射时间、剂量、部位及次数与SG组小鼠一致。分别于损伤前、损伤后1 d和损伤后16 d,采用平衡木实验评价小鼠运动协调能力,网屏实验评价小鼠肢体肌力,圆筒实验评价小鼠患肢使用率;损伤后16 d,采用免疫印迹法检测2组小鼠梗死灶周围区域中突触蛋白1(SYN1)表达水平。结果:平衡木实验,损伤后1 d,2组小鼠运动协调能力评分均明显低于损伤前(P<0.01);损伤后16 d,2组小鼠运动协调能力评分均高于损伤后1 d(P<0.01),SG组小鼠运动协调能力评分较SS组升高(P<0.05)。网屏实验,损伤后1 d,2组小鼠倒置抓握网屏时的停留时间明显短于损伤前(P<0.01);损伤后16 d,2组小鼠停留时间均优于损伤后1 d(P<0.01);与SS组比较,SG组小鼠倒置抓握网屏时的停留时间明显延长(P<0.05)。圆筒实验,损伤后1 d,2组小鼠患侧肢体使用率明显低于损伤前(P<0.01);损伤后16 d,2组小鼠患侧肢体使用率均高于损伤后1 d(P<0.01),其中SG组小鼠较SS组患侧肢体使用率更高(P<0.05)。免疫印迹法检测,与SS组比较,SG组小鼠梗死灶周围区域中SYN1表达水平明显升高(P<0.05)。结论:重组人生长激素能够改善局灶性脑缺血再灌注小鼠的运动功能,其机制可能与促进梗死灶周围区域中SYN1的表达有关。Objective:To observe the effect of recombinant human growth hormone on motor function in the mice with focal cerebral ischemia-reperfusion,and to explore its possible mechanism.Methods:A total of 24 C57BL/6J mice were selected to establish the cerebral ischemia-reperfusion injury models by the modified Zea-Longa suture method,and they were randomly divided into growth hormone treatment group(cerebral ischemia-reperfusion+growth hormone,SG group,n=12)and control group(cerebral ischemia-reperfusion+saline,SS group,n=12).A total of 48 h after injury,the mice in SG group were injected subcutaneously with the recombinant human growth hormone at the dose of 1.4 mg·kg^(-1)·d^(-1) for 14 d,and the mice in SS group were injected with saline,and the injection time,dose,location and times were consistent with the mice in SG group.Beam balance test was used to evaluate the motor coordination abilities of the mice before injury,1 d after injury,and 16 d after injury;screen test was used to evaluate the muscle strengths of the mice,and the cylinder test was used to evaluate the utilization rates of the affected limb of the mice;the expression levels of synapsin1(SYN1)in peri-infarct region of the mice in two groups were detected by Western blotting method 16 d after injury.Results:The beam balance test results showed that compared with before injury,the scores of motor coordination abilities of the mice in two groups 1 d after injury were decreased(P<0.01);the scores of motor coordination abilities of the mice in two groups 16 d after injury were higher than those 1 d after injury(P<0.01),and the score of motor coordination ability of the mice in SG group was higher than that in SS group(P<0.05).The screen test results showed that compared with before injury,the residence time when grasping the screen upside down of the mice in two groups was significantly shortened 1 d after injury(P<0.01),and the residence time of the mice in two groups 16 d after injury was longer than that 1 d after injury(P<0.01);compared with SS group,

关 键 词：重组人生长激素 脑缺血再灌注 运动功能障碍 突触蛋白1 神经修复 

分 类 号：R45[医药卫生—治疗学] R493[医药卫生—临床医学]