miR-328-3p靶向PINK1基因对胃癌细胞增殖侵袭的影响机制研究  

INFLUENCING MECHANISM OF MIR-328-3P TARGETING PINK1 GENE ON PROLIFERATION AND INVASION OF GASTRIC CANCER CELLS

作　　者：张辉[1] 靳伟民[1] 韩一梅 金瑞[1] ZHANG Hui;JIN Weimin;HAN Yimei;JIN Rui(Hanzhong Central Hospital,Hanzhong 723000,China)

机构地区：[1]陕西省汉中市中心医院,723000

出　　处：《中国煤炭工业医学杂志》2022年第2期121-126,共6页Chinese Journal of Coal Industry Medicine

基　　金：陕西省自然科学基础研究计划项目(编号:2021JM-526);汉中市中心医院院级科研基金项目(编号:YK1929)。

摘　　要：目的探究miR-328-3p靶向PINK1基因对胃癌(GC)细胞增殖侵袭的影响机制,旨在为GC新型靶向治疗提供参考。方法收集2019年1月—2021年1月汉中市中心医院收治的91例GC患者癌组织及癌旁组织新鲜标本(冻存于液氮罐内),对MGC-803胃癌细胞株进行培养,依据细胞转染情况分为对照组(正常培养的MGC-803细胞)、miR-328-3p组(转染miR-328-3p模拟物)、PINK1-pcDNA组(转染PINK1-pcDNA)、miR-328-3p+PINK1-pcDNA组(miR-328-3p和PINK1-pcDNA共转染)。RT-PCR检测胃癌癌组织、癌旁组织中miR-328-3p表达情况,对比各组PINK1蛋白及mRNA表达量、细胞增殖情况、细胞增殖相关蛋白(Cyclin B1、Cyclin D1)表达、细胞侵袭情况。结果GC患者癌组织中miR-328-3p相对表达量明显低于癌旁组织(P<0.05)。PINK1-pcDNA组PINK1蛋白及mRNA表达量明显高于对照组、miR-328-3p+PINK1-pcDNA组(P<0.05)。miR-328-3p组细胞增殖率、细胞增殖相关蛋白Cyclin B1、Cyclin D1表达水平、穿膜细胞数较对照组、PINK1-pcDNA组、miR-328-3p+PINK1-pcDNA组明显低,对照组、miR-328-3p+PINK1-pcDNA组细胞增殖率、细胞增殖相关蛋白Cyclin B1、Cyclin D1表达水平、穿膜细胞数较PINK1-pcDNA组明显低,差异有统计学意义(P<0.05)。结论miR-328-3p可通过靶向调控PINK1基因,明显抑制GC细胞增殖侵袭,为GC的新型靶向治疗提供必要的参考。Objective To explore the influencing mechanism of miR-328-3 p targeting PINK1 gene on proliferation and invasion of gastric cancer(GC)cells,aiming to provide reference for new targeted therapy of GC.Methods The fresh specimens of cancer tissues and adjacent tissues were collected from ninety-one patients with GC admitted to Hanzhong Central Hospital between January 2019 and January 2021,and cryopreserved in liquid nitrogen tanks.MGC-803 gastric cancer cell line was cultured.According to the status of cell transfection,the cells were divided into control group(normally cultured MGC-803 cells),miR-328-3 p group(transfected with miR-328-3 p mimic),PINK1-pcDNA group(transfected with PINK1-pcDNA),miR-328-3 p+PINK1-pcDNA group(co-transfected with miR-328-3 p and PINK1-pcDNA).The expression of miR-328-3 p in gastric cancer tissues and adjacent tissues was detected by RT-PCR.The expression levels of PINK1 protein and mRNA,cell proliferation,the expression of cell proliferation-related proteins(Cyclin B1,Cyclin D1),and status of cell invasion were compared among the groups.Results The relative expression level of miR-328-3 p in GC tissues was significantly lower than that in adjacent tissues(P<0.05).The expression levels of PINK1 protein and mRNA in the PINK1-pcDNA group were significantly higher than those in the control group and the miR-328-3 p+PINK1-pcDNA group(P<0.05).The cell proliferation rate,expression levels of cell proliferation-related proteins(Cyclin B1,Cyclin D1),and transmembrane cell count in the miR-328-3 p group were significantly lower than those in the other 3 groups.Meanwhile,cell proliferation rate,expression levels of cell proliferation-related proteins(Cyclin B1,Cyclin D1),and transmembrane cell count in the control group and the miR-328-3 p+PINK1-pcDNA group were significantly lower than those in the PINK1-pcDNA group(P<0.05).Conclusion miR-328-3 p can significantly inhibit the proliferation and invasion of GC cells through targeting PINK1 gene,which provides reference for new targeted therapy of

关 键 词：胃癌 miR-328-3p PINK1 增殖 侵袭 

分 类 号：R34[医药卫生—基础医学]