N6-甲基腺嘌呤修饰与环状RNA  

N6-methyladenine and circular RNA

作　　者：胡潇 靳腾喻 王仲璇 刘悦林 尹铮 孙绍光 HU Xiao;JIN Teng-yu;WANG Zhong-xuan;LIU Yue-lin;YIN Zheng;SUN Shao-guang(Department of Biochemistry and Molecular Biology,College of Basic Medicine,Hebei Medical University,Cardiovascular Medical Science Center,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学基础医学院生物化学与分子生物学教研室心血管医学研究中心,河北石家庄050017

出　　处：《生物技术》2022年第2期239-245,199,共8页Biotechnology

基　　金：国家自然科学基金项目(82170439,81670273,81200215);河北省自然科学基金项目(H2021206399,H2019206150);大学生创新性实验计划项目(USIP2020018,USIP2020040)。

摘　　要：N6-甲基腺嘌呤(N6-methyladenosine,m6A)是发生在腺嘌呤第6位氮原子上的甲基化修饰,广泛存在于多种真核生物的RNA中。环状RNA(circular RNA,circRNA)是一类表达稳定的非编码RNA。该文分别从m6A影响circRNA的形成和翻译、降解、出核、先天免疫,通过下游分子影响circRNA的方面,以及circRNA竞争性结合三种m6A相关酶影响其他RNA的m6A修饰,海绵吸附微小RNA(microRNA,miRNA)靶向m6A相关酶,调控甲基转移酶的表达,结合去甲基化酶的mRNA促进其表达,增强去甲基化酶与mRNA的相互作用,结合并参与泛素化降解m6A结合蛋白的方面,及其他间接联系方面总结归纳了二者之间的作用机制,以期为研究m6A与circRNA相互影响的机制提供参考。N6-methyladenine(m6 A) refers to the methylation modification on the 6 th nitrogen atom of adenine,which is widely found in many eukaryotes′ RNAs.Circular RNA(circRNA)is a kind of non-coding RNA with stable expression.This review summarizes the mechanism of the interaction between m6 A and circRNA,from the aspects of m6 A affecting circRNA′s formation and translation,degradation,nucleation,innate immunity,affecting circRNA through downstream molecules,and circRNA competitively binding three m6 A related enzymes to affect m6 A modification of other RNAs,acting like a sponge to absorb microRNAs(miRNAs)targeting m6 A related enzymes,regulating methyltransferase expression,binding demethylase mRNA to promote its expression,enhancing the interaction between demethylase and mRNA,promoting circRNA binding and participating in ubiquitination degradation of m6 A-binding protein,and other indirect links,in order to provide references for further studies.

关 键 词：环状RNA N6-甲基腺嘌呤 甲基转移酶 去甲基化酶 甲基结合蛋白 

分 类 号：Q522[生物学—生物化学]