广西辣椒病毒的sRNA深度测序和RT-PCR鉴定  

Identification of Viruses Infecting Peppers in Guangxi by Small RNA Deep Sequencing and RT-PCR

作　　者：龚明霞 赵虎 王萌 吴星 赵曾菁 何志 黄金梅 孟生德 王日升 GONG Mingxia;ZHAO Hu;WANG Meng;WU Xing;ZHAO Zengjing;HE Zhi;HUANG Jinmei;MENG Shengde;WANG Risheng(Guangxi Key Laboratory of Vegetable Breeding and New Technology Development,Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Gansu Dunhuang Seed Industry Group Co.Ltd,Jiuquan,Gansu 735008,China)

机构地区：[1]广西壮族自治区农业科学院蔬菜研究所,广西蔬菜育种与新技术研究重点实验室,南宁530007 [2]甘肃省敦煌种业集团股份有限公司,甘肃酒泉735008

出　　处：《园艺学报》2022年第5期1060-1072,共13页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(32060687);广西科技重大专项(桂科AA17204026);广西自然科学基金项目(2019 GXNSFAA 185039);广西农业科学院基本科研业务专项(桂农科2019M30)。

摘　　要：采用小RNA(sRNA)深度测序技术,结合RT-PCR技术对广西9个辣椒主产区具有典型病毒病症状的病样进行检测,共发现10种病毒,其中小米椒内源RNA病毒1(Capsicum frutescens endornavirus 1,CFEV 1)、辣椒潜隐病毒2(pepper cryptic virus 2,PCV 2)、烟草轻型绿花叶病毒(tobacco mild green mosaic virus,TMGMV)和辣椒黄脉病毒(pepper vein yellows virus,PeVYV)是在广西辣椒上首次发现,且CFEV 1在中国是首次发现。10种病毒在75份辣椒病样中的总检出率为8.00%~66.67%,前5位依次为辣椒脉斑驳病毒(chilli veinal mottle virus,ChiVMV,66.67%)、甜椒脉斑驳病毒(pepper veinal mottle virus,PVMV,62.67%)、黄瓜花叶病毒(cucumber mosaic virus,CMV,41.33%)、CFEV 1(32.0%)以及PCV 2(26.67%)。ChiVMV、PVMV、CMV和CFEV 1在广西7~9个辣椒主产区广泛分布,推测为优势病毒。除桂林外,其他8个辣椒主产区均含以上4种优势病毒中的1~3种,但在桂林,这4种病毒的检出率偏低或检不出,其优势病毒种类是PMMoV和PCV 2。广西辣椒病毒病复合侵染率为94.66%,3种(检出率42.67%)和4种(检出率24.00%)病毒复合侵染是主要形式。3种复合侵染中以ChiVMV+PVMV+CMV和ChiVMV+CFEV 1+ChiRSV占比最高(均为18.75%),4种复合侵染中以ChiVMV+PVMV+CMV+CFEV 1占比最高(16.67%)。We analysed diseased pepper samples with typical viral symptoms collected from nine of major pepper growing areas in Guangxi through small RNA(sRNA)deep sequencing technology and RT-PCR. The results showed that ten viruses were identified,among which,Capsicum frutescens endornavirus 1(CFEV 1),pepper cryptic virus 2(PCV 2),tobacco mild green mosaic virus(TMGMV)and pepper vein yellows virus(Pe VYV)were detected on pepper for the first time in Guangxi,and CFEV 1 was also detected for the first time in China. The total detection rates of ten viruses in 75 diseased pepper samples were 8.00%–66.67%. The top five viruses were chilli veinal mottle virus(ChiVMV,66.67%),pepper veinal mottle virus(PVMV,62.67%),cucumber mosaic virus(CMV,41.33%),CFEV 1(32.0%)and PCV 2(26.67%). ChiVMV,PVMV,CMV and CFEV 1 were found to be widely distributed across seven to nine of the major pepper growing areas of Guangxi,which were speculated to be the dominant viruses infecting peppers in Guangxi. Except Guilin,other eight regions contained at least one to three of the four dominant viruses. However,in Guilin,the detection rates of these four dominant viruses were relatively low or negligible,and the dominant viruses were PMMoV and PCV 2. The mixed infection rate of pepper virus disease was up to 94.66% in Guangxi,and the mixed infection were mostly caused by a complex of three(detection rate 42.67%)or four viruses(detection rate 24.00%)in Guangxi. Mixed infection of three viruses were predominantly caused by ChiVMV+PVMV+CMV and ChiVMV+CFEV 1+ChiRSV both with detection rate of 18.75%,that of four viruses was mostly caused by ChiVMV+PVMV+CMV+CFEV 1 with detection rate of 16.67%.

关 键 词：辣椒 病毒病 小RNA 深度测序 RT-PCR 分子鉴定 

分 类 号：S641.3[农业科学—蔬菜学]