63份柚类资源S基因型鉴定  

Identification of S-genotypes of 63 Pummelo Germplasm Resources

作　　者：韦壮敏 魏斯佳 陈鹏[2] 胡健兵 汤雨晴 叶俊丽[1] 李先信[2] 邓秀新[1] 柴利军[1] WEI Zhuangmin;WEI Sijia;CHEN Peng;HU Jianbing;TANG Yuqing;YE Junli;LI Xianxin;DENG Xiuxin;CHAI Lijun(Key Laboratory of Horticultural Plant Biology(Ministry of Education),Huazhong Agricultural University,Wuhan 430070,China;Institute of Horticulture,Hunan Academy of Agricultural Sciences,Changsha 410125,China;Institute of Horticulture,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;Guangxi Subtropical Crops Research Institute,Nanning 530001,China)

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室,武汉430070 [2]湖南省农业科学院园艺研究所,长沙410125 [3]江西省农业科学院园艺研究所,南昌330200 [4]广西壮族自治区亚热带作物研究所,南宁530001

出　　处：《园艺学报》2022年第5期1111-1120,共10页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2021YFD1200200);国家自然科学基金项目(32122075,32072523);华中农业大学自主科技创新基金项目(2662019PY044)。

摘　　要：以‘晚白柚’等63份柚类资源为材料基础,通过RNA-seq和序列特征分析发掘新的S-RNase,并结合前期已有的9个S-RNase基因信息,设计21对特异性引物,扩增鉴定63份柚类资源的S基因型。结果发掘到12个新的S-RNase,按顺序分别命名为S_(10)-RNase~S_(21)-RNase。特异扩增结果显示:鉴定的63份材料中,有59份材料得到完整的S基因型,4份仅鉴定到1个S-RNase基因,其中S_(2)-RNase扩增频率最高,达34.92%(22/63),S_(4)-RNase和S_(6)-RNase在63份材料中均未扩增出。结果还显示许多品种具有相同的S基因型。Based on 63 pummelo germplasm resources,new S-RNases were identified by RNA-seq and sequence analysis.Twenty-one pairs of specific primers were designed to amplify and identify the S-genotypes of 63 pummelo germplasm resources.Results showed twelve new S-RNases were found,named S_(10)-RNase–S_(21)-RNase in sequence.Specific amplification showed that 59 out of 63 materials were identified with complete S-genotype,only one S-RNase gene was identified in four materials.Among them,S_(2)-RNase was amplified in 22 materials with the highest frequency of 34.92%(22/63),while S_(4)-RNase and S_(6)-RNase were not amplified in the 63 materials.The results also showed that many varieties had the same S-genotype.

关 键 词：柑橘 S基因型 自交不亲和 特异性引物 

分 类 号：S666.3[农业科学—果树学]