Simultaneous quantification of 18 bioactive constituents in Ziziphus jujuba fruits by HPLC coupled with a chemometric method  

作　　者：Tianyi Wang Sen Guo Xiameng Ren Junfeng Du Lu Bai Xueqin Cui Chi-Tang Ho Naisheng Bai 

机构地区：[1]College of Food Science and Technology,Northwest University,Xi’an 710069,China [2]College of Chemical Engineering,Northwest University,229 Taibai North Road,Xi’an 710069,China [3]Shaanxi Family Forestry Bureau,Shaanxi Jiaxian Development and Reform and Science and Technology Bureau,Yulin 719200,China [4]Department of Food Science,Rutgers University,New Brunswick 08901,USA

出　　处：《Food Science and Human Wellness》2022年第4期771-780,共10页食品科学与人类健康（英文）

基　　金：supported by a grant from Desert Control Research Institute of Shaanxi Province(No.203130012);National Natural Science Foundation of China(No.31570348)。

摘　　要：The fruit of Ziziphus jujuba Mill., known as Hongzao(or Hong-Zao) in Chinese and cultivated in China for more than 4 000 years, has shown to have hepatoprotective property. In previous study, we have isolated and identified 27 known compounds from Z. jujuba fruits, which demonstrated anti-tumor activity. In this study, a high-performance liquid chromatography-diode-array detection-mass spectrometry(HPLC-DAD-MS) method was successfully applied to the simultaneous characterization and quantitation of 18 constituents in 28 Z. jujuba samples, comprised of 12 cultivars from different regions in China, by comparing their HPLC retention times, MS spectra, UV spectra, and NMR data with those of reference compounds. The quantitative method was validated with excellent linearity(R^(2 )> 0.999 1), preferable intra-and inter-day precisions(RSD < 2.78%), and good recoveries(94.96%–102.65%). The content variation of 18 compounds was analyzed by a chemometric method(hierarchical cluster analysis). In addition, these constituents showed protection against carbon tetrachloride(CCl_(4)) intoxicated Hep G2 cell lines by decreasing lactic dehydrogenase(LDH) levels. Results in this study illustrated that the content of all 18 compounds examined has significant difference and variation among cultivars and extracts. The proposed method can serve as a prerequisite for quality control of bioactive compounds in Z. jujuba products.

关 键 词：Ziziphus jujuba HPLC-DAD-MS Quantitative analysis Hierarchical cluster analysis HepG2 cells 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]