Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study  

作　　者：Junjie Lin Huan Xiang Dongxiao Sun-Waterhouse Chun Cui Wei Wang 

机构地区：[1]College of Food Science and Technology,South China University of Technology,Guangzhou 510640,China [2]Guangdong Wei-Wei Biotechnology Co.,Ltd,Guangzhou 510640,China

出　　处：《Food Science and Human Wellness》2022年第4期1028-1035,共8页食品科学与人类健康（英文）

基　　金：the financial support from the National Natural Science Foundation of China (No. 31201416);Science and Technology Research Program of Guangdong Province (No. 2017A01010502)。

摘　　要：Seabuckthorn seed meal(SSM) is a waste of oil extraction industry that rich in protein. In order to seek suitable protein extraction method, three different deep eutectic solvents(DESs)(including choline chlorideglycerol, choline chloride-oxalic acid and choline chloride-urea) were developed for extracting protein from SSM and compared with alkaline. Result indicated that alkaline could effectively extract 56.9% protein from SSM and its protein content was 73.1%, higher than DES at 31.0%-41.4% and 64.3%-67.5%, respectively. However, compared to alkali, DES led to a product with less β-sheet, more β-turn, more essential amino acids, higher total amino acid content, especially choline chloride-urea which extracted protein showing an integrated and similar protein weight distribution compared to SSM. Also, this protein extracted chloride-urea showed a highest digestibility in vitro(by pepsin)(54.2%). These results indicated that choline chloride-urea extraction is better than alkaline extraction for SSM.

关 键 词：Seabuckthorn seed meal Protein extraction Deep eutectic solvent Alkaline extraction and acid precipitation Amino acids 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]