黄芩茎叶-虎杖配伍对急性肺损伤大鼠TRPV1表达及炎性细胞因子的影响  

Effects of compatibility of Scutellaria baicalensis stems and Polygonum cuspidatum on TRPV1 expression and inflammatory cytokines in rats with acute lung injury

作　　者：屈新亮 段广靖 赵博 谢锋 王斌[1] 高峰[1] 卫培峰[1] 李敏[1] QU Xin-liang;DUAN Guang-jing;ZHAO Bo;XIE Feng;WANG Bin;GAO Feng;WEI Pei-feng;LI Min(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046

出　　处：《海南医学院学报》2022年第11期809-814,共6页Journal of Hainan Medical University

基　　金：陕西中医药大学学科创新团队资助项目(2019-QN02);陕西省教育厅项目(黄芩茎叶和忍冬藤抗畜禽感染产品研究与开发)(20JC012)。

摘　　要：目的:通过观察TRPV1表达和炎性细胞因子等指标研究黄芩茎叶-虎杖配伍对脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠的保护作用。方法:将48只SD雄性大鼠随机分为6组:对照组、模型组、地塞米松组(5.0 mg/kg)、黄芩茎叶-虎杖低、中、高剂量(3.5、7.0、14.0 g/kg)组。给药组灌胃7 d,对照组和模型组给予等量0.9%氯化钠。第8天,除对照组外,其余各组大鼠经尾静脉注射8 mg/kg的LPS诱导ALI模型。造模6 h后采集大鼠肺组织,计大鼠肺组织湿/干重比值(W/D);HE染色观察肺组织病理变化;测定肺泡灌洗液(BALF)中的肿瘤坏死因子-α(TNF-α)、白介素-1β(1L-1β)的含量及血清中超氧化物歧化酶(SOD)的活力;检测大鼠肺组织中TRPV1受体的mRNA及蛋白表达水平。结果:与模型组相比,黄芩茎叶-虎杖可使肺组织结构破坏及出血状态明显减轻,W/D值、TNF-α、IL-1β含量均降低,SOD水平提高,TRPV1受体的mRNA与蛋白表达均下调。结论:黄芩茎叶-虎杖配伍对急性肺损伤大鼠有保护作用,其作用机制可能与下调TRPV1表达,抑制炎性细胞水平TNF-α、IL-1β有关。Objective:The protective effect of Scutellaria baicalensis(S.baicalensis)stems and Polygonum cuspidatum(P.cuspidatum)compatibility on lipopolysaccharide(LPS)-induced acute lung injury(ALI)in rats was studied by observing the expression of TRPV1 and inflammatory cytokines.Methods:A total of 48 male SD rats were randomly divided into 6 groups:control group,model group,dexamethasone group(5.0 mg/kg)and S.baicalensis stems-P.cuspidatum(3.5,7.0 and 14.0 g/kg).The administration group was gavaged for 7 days,and the control group and model group were given the same amount of 0.9%sodium chloride.On the 8th day,except the control group,rats in other groups were injected with 8 mg/kg LPS through caudal vein to induce Ali model.Take the rat lung tissue 6 hours after modeling,and calculate the wet/dry weight ratio(W/D)of the rat lung tissue;HE staining to observe the pathological changes of lung tissue;Determine the content of tumor necrosis factor-α(TNF-α)and interleukin-1β(1L-1β)in alveolar lavage fluid(BALF)and the activity of superoxide dismutase(SOD)in serum;Detect the mRNA and protein expression levels of TRPV1 receptor in rat lung tissue.Results:Compared with the model group,S.baicalen⁃sis stems-P.cuspidatum can significantly reduce the damage of lung tissue structure and bleeding state,W/D value and TNF-α、IL-1βThe content of TRPV1 decreased,the level of SOD increased,and the mRNA and protein expression of TRPV1 receptor decreased.Conclusion:The combination of S.baicalensis stems-P.cuspidatum has a protective effect on acute lung injury in rats,and its mechanism may be related to down-regulating the expression of TRPV1 and inhibiting the levels of TNF-αand IL-1βin inflammatory cells.

关 键 词：黄芩茎叶 虎杖 急性肺损伤 炎症反应 TRPV1受体 

分 类 号：R285.5[医药卫生—中药学]