The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris  

作　　者：Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-Ying Li Ying-Chen Lu Fang-Rong Chang 

机构地区：[1]Graduate Institute of Natural Products,Kaohsiung Medical University,Kaohsiung 807,Taiwan,China [2]Graduate Institute of Integrated Medicine,China Medical University,Taichung 404,Taiwan,China [3]Chinese Medicine Research and Development Center,China Medical University Hospital,Taichung 404,Taiwan,China [4]Department of Food Science and Nutrition,Meiho University,Pingtung 912,Taiwan,China [5]Department of Food Science,National Chiayi University,Chiayi City 600,Taiwan,China [6]Drug Development and Value Creation Research Center,Kaohsiung Medical University,Kaohsiung 807,Taiwan,China [7]Department of Medical Research,Kaohsiung Medical University Hospital,Kaohsiung Medical University,Kaohsiung 807,Taiwan,China [8]Department of Marine Biotechnology and Resources,National Sun Yat-sen University,Kaohsiung 804,Taiwan,China

出　　处：《Food Science and Human Wellness》2022年第5期1240-1251,共12页食品科学与人类健康（英文）

基　　金：supported by grants from the Ministry of Science and Technology of Taiwan(Grant number:MOST 106-2320-B-037008-MY2,MOST 108-2320-B-037-022-MY3,108-2811-B-037-511,and 109-2927-I-037-502);funded by the Drug Development and Value Creation Research Center,Kaohsiung Medical University;Department of Medical Research,Kaohsiung Medical University Hospital(Grant number:KMU-TC108A03-11)。

摘　　要：This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two ways.The protoplasts of A.cinnamomea were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm,and the protoplasts of C.militaris were inactivated by UV radiation to invalidate their genome function,then they were fused under optimal conditions to get a fusion rate as(7.42±0.8)×10^(-6) fusants/mL;the new fusants were abbreviated as Ac-Cm.On the other hand,when A.cinnamomea and C.militaris were treated with heat and UV oppositely using similar experiments,the fusion rate was(9.70±0.68)×10^(-5) fusants/mL,and the new fusants were abbreviated as Cm-Ac.We selected each of two best-growing fused colonies Ac-Cm-1,Ac-Cm-2,Cm-Ac-1,and Cm-Ac-2,together with parental A.cinnamomea and C.militaris,and studied their morphology,growth antagonism tests,and genetic relationships by 18 S rRNA sequencing.In comparison with the initial cultures of 4 fusants,the yields of adenosine,biomass,cordycepic acid,cordycepin,total polysaccharide,and total triterpenoids were increased up 1.305-50.1563 times in the optimal medium conditions.For gene stability tests,those of the four fusants and their outputs were stabilized within 10 generations.

关 键 词：Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite 

分 类 号：S567.3[农业科学—中草药栽培]