Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection ofβ-lactoglobulin allergen in milk products  

作　　者：Mingfei Pan Liping Hong Jingying Yang Xiaoqian Xie Kaixin Liu Shuo Wang 

机构地区：[1]Key Laboratory of Food Nutrition and Safety,Ministry of Education of China,Tianjin University of Science and Technology,Tianjin 300457,China [2]State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457,China

出　　处：《Food Science and Human Wellness》2022年第5期1315-1321,共7页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(31972147);Project of Tianjin Science and Technology Plan(19PTSYJC00050);the Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology(SKLFNS-KF-202011);Project program of Key Laboratory of Food Nutrition and Safety,Ministry of Education,Tianjin Key Laboratory of Food Nutrition and Safety,China(JYB202002)。

摘　　要：In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk products.Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment,amount of anti-β-LG antibody and regeneration reagent were optimized in detail.Under optimal conditions,the proposed QCM immunochip exhibited good recognition of β-LG,with a calibration curve of ΔF=12.877 C_(β-LG)^(0.4809)(R^(2)=0.9982)and limit of detection of 0.04μg/mL.Additionally,this portable QCM immunochip had good stability,high specificity,and no obvious cross-reaction to three other milk proteins(α-casein,α-lactalbumin,and lactoferrin).It could compete a qualitative measurement within5 min,and could be reused at least ten times.In the β-LG analysis of actual milk samples,the developed QCM immunochip yielded reliable and accurate results,which correlated strongly with those from the standard HPLC method(R^(2)=0.9969).Thus,the portable,stable,and reproducible QCM immunochip developed in this study allowed the rapid,cost-effectively and sensitively measure theβ-LG in milk products.

关 键 词：β-Lactoglobulin allergen Quartz crystal microbalance Immunochip Milk products 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] TN492[轻工技术与工程—食品科学与工程]