丁酸梭菌对主要商业饲用益生菌抑制作用的研究  

Study on Inhibitory Effect of Clostridium butyricum on Main Commercial Feed Probiotics

作　　者：王绍斌 张海鹏 高映红 王配[1] 肖明霞 王乙茹 PHOUTHAPANE Vanhnaseng 程志斌[1] WANG Shaobin;ZHANG Haipeng;GAO Yinghong;WANG Pei;XIAO Mingxia;WANG Yiru;PHOUTHAPANE Vanhnaseng;CHENG Zhibin(Yunnan Agricultural University,Kunming 650201,China;Animal Health Supervision Institute of Hongta District of Yuxi,Yuxi 653100,Yunnan China;Yunling Guangda Breeding Poultry and Feed Limited Company,Kunming 650200,China;Dazhou Vocational and Technical College,Dazhou 635001,Sichuan China;Department of Livestock and Fisheries,Ministry of Agriculture and Forestry of Laos,Vientiane 6644,Laos)

机构地区：[1]云南农业大学,昆明650201 [2]玉溪市红塔区动物卫生监督所,云南玉溪653100 [3]昆明云岭广大种禽饲料有限公司,昆明650200 [4]达州职业技术学院,四川达州635001 [5]老挝农林部畜牧与水产局,老挝万象6644

出　　处：《饲料博览》2022年第3期8-12,共5页Feed Review

基　　金：云南省创新团队项目(2016ZA008);云南农业大学博士科研启动基金(A2032002455)。

摘　　要：研究旨在体外评估益生菌丁酸梭菌对主要商业饲用益生菌的抑制作用。采用牛津杯法,以抑菌圈直径判定丁酸梭菌对6个主要商业饲用益生菌(凝结芽孢杆菌、枯草芽孢杆菌、地衣芽孢杆菌、屎肠球菌、粪肠球菌、产朊假丝酵母)的抑制作用。结果显示:丁酸梭菌对凝结芽孢杆菌的抑制作用达到“高敏感”,对地衣芽孢杆菌的抑制作用为“中敏感”,对枯草芽孢杆菌的抑制作用为“低敏感”,对屎肠球菌、粪肠球菌、产朊假丝酵母的抑制作用均判定为“不敏感”。综上,基于丁酸梭菌对其他商业饲用益生菌存在的抑制作用,建议复合饲用益生菌产品要充分考虑丁酸梭菌与其他饲用益生菌之间可能的拮抗效应。Evaluate the inhibitory effect of Clostridium butyricum on the commercial feed probiotics in vitro were conducted in this study.The inhibitory effect of Clostridium butyricum on six commercial probiotics(Bacillus coagulans,Bacillus subtilis,Bacillus licheniformis,Enterococcus faecium,Enterococcus faecalis and Candida utili)were determined by comparing the diameter of inhibition zone(DIZ)using Oxford cup method in vitro.The results showed that Clostridium butyricum was"highly sensitive"to Bacillus coagulans,"moderately sensitive"to Bacillus licheniformis,"low sensitive"to Bacillus subtilis,and"insensitive"to Enterococcus faecium,Enterococcus faecalis and Candida utilis.In conclusion,based on the inhibitory effect of Clostridium butyricum on other commercial feed probiotics,it was suggested that the possible antagonistic effect between Clostridium butyricum and other feed probiotics should be fully considered.

关 键 词：丁酸梭菌 抑菌作用 商业饲用益生菌 牛津杯法 

分 类 号：S816.3[农业科学—饲料科学]