基于金属有机框架载体的免疫球蛋白G印迹材料的构筑及其识别性能研究  

Study on the Preparation and Recognition Performance of Immunoglobulin G Imprinted Material Based on Metal Organic Framework

作　　者：宋文琦 杨雨璇 刘文倩 钱立伟 Wen-qi Song;Yu-xuan Yang;Wen-qian Liu;Li-wei Qian(School of Science,Xijing University,Xi'an 710123;College of Bioresources Chemical and Materials Engineering,Shaanxi University of Science and Technology,Xi'an 710021;School of Chemistry and Chemical Engineering,Northwestern Polytechnical University,Xi'an 710072)

机构地区：[1]西京学院理学院,西安710123 [2]陕西科技大学轻工科学与工程学院,西安710021 [3]西北工业大学化学与化工学院,西安710072

出　　处：《高分子学报》2022年第6期653-662,共10页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号21805177);陕西省重点研发计划项目(项目号2021GY-240)资助.

摘　　要：以人免疫球蛋白G(IgG)作为模板分子,利用模板固定化与表面印迹技术相结合策略制备新型、高效的蛋白印迹材料.为了增加印迹材料的分离效果,金属有机框架UIO-66被作为模板蛋白固定化的纳米载体.同时为了提高印迹材料的识别性,在其制备中加入了两性离子单体.研究结果表明,UIO-66晶体呈现出规则的正八面体形状,由它所制备的印迹材料(UIO-66@MIPs)展现出优异的比表面积(362.5 m^(2)/g).此外,通过对印迹聚合物制备过程中两性离子单体用量的优化,发现其占功能单体总质量20%时,可以获得最佳的识别性.吸附实验研究结果表明,UIO-66@MIPs可在浓度为2.0 mg/mL的Ig G溶液中达到饱和吸附,其饱和吸附量可达217.4 mg/g,最大印迹因子为2.48,吸附平衡时间为50 min.另外,选择性和竞争性吸附实验表明,与非印迹材料相比,UIO-66@MIPs对Ig G具有更强的亲合能力,并可以从混合蛋白溶液中高效分离Ig G.本文的研究结果将为构筑高性能Ig G识别材料提供新的思路,对设计高效、廉价的新型冠状病毒检测试剂奠定理论基础.In this work,human immunoglobulin G(IgG)is selected as the template,and the strategy of combining template immobilization and surface imprinting technology is used to prepare a new and efficient protein imprinting material(MIPs).In order to increase the separation effect of MIPs,the metal organic framework(UIO-66)is fabricated and used as a carrier for protein immobilization.Meanwhile,in order to improve the recognition of imprinting materials,zwitterionic monomers participate in the preparation of MIPs to improve their recognition ability.The research results showed that the UIO-66 crystal presented a regular octahedral shape,and the as-prepared MIPs exhibited an excellent specific surface area(362.5 m^(2)/g).In addition,by optimizing the amount of zwitterionic monomer,it was found that the best recognition could be obtained when it accounted for 20% of the total mass of the functional monomer.The adsorption experiments also demonstrated that UIO-66@MIPs could achieve saturated adsorption in an Ig G solution with a concentration of 2.0 mg/mL,and its saturated adsorption capacity reached 217.4 mg/g with the imprinting factor of 2.48.Furthermore,the adsorption equilibrium time of UIO-66@MIPs was about 50 min.Moreover,the specific recognition experiment of UIO-66@MIPs showed that compared with non-imprinted materials,it could separate Ig G more effectively from the protein mixture solution,proving their excellent specific recognition capability.

关 键 词：蛋白质印迹技术 金属有机框架 两性离子单体 

分 类 号：O631.3[理学—高分子化学] O647.3[理学—化学] R318.08[医药卫生—生物医学工程]