Fabrication and characterization of hierarchical porous Ni2+doped hydroxyapatite microspheres and their enhanced protein adsorption capacity  

作　　者：Yaling Li Hao Ai Liangzhi Qiao Yinghong Wang Kaifeng Du 

机构地区：[1]Department of Pharmaceutical&Biological Engineering,School of Chemical Engineering,Sichuan University,Chengdu 610065,China

出　　处：《Chinese Journal of Chemical Engineering》2022年第5期238-247,共10页中国化学工程学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(21676170)。

摘　　要：Hydroxyapatite(HAP)is a common bio-adsorbent,which performance depends heavily upon its morphology and microporous structure.In this study,a novel synthesis strategy was proposed for hierarchical porous HAP microspheres by a simple"one-pot"hydrothermal reaction.In the strategy,L-glutamic acid serves as soft template to modulate the morphology and inner crystalline of HAP.To evaluate the application potential,doping Ni^(2+) on hierarchical porous HAP microspheres gives metal chelated affinity adsorbents.The prepared adsorbents show a perfect spherical shape,particles size of 96.6μm,relatively specific surface area of 48.5 m^(2)·g^(-1) and hierarchical pores(mesopores:4 nm and macropores:53 nm).By the adsorption evaluation,it reveals that the Ni^(2+)-HAP adsorbents have high adsorption capacities of275.11 and 97.55 m^(2)·g^(-1) for hemoglobin and bovine serum albumin,respectively,which is comparable to other similar adsorbent.Therefore,this work provides a promising method for high-efficiency hydroxyapatite microspheres for proteins purification.

关 键 词：Hierarchical porous hydroxyapatite MICROSPHERES Templates Metal doping Proteins adsorption 

分 类 号：TQ424[化学工程]