肝经郁热型干眼患者的泪液蛋白质组学研究  

Study on the proteomics of tears in dry eye patients with liver meridian stagnation heat

作　　者：刘培 蒋鹏飞[1,2] 梁昊 杨多[3] 谭亢 颜春薇 彭俊 彭清华[1,2] LIU Pei;JIANG Peng-fei;LIANG Hao;YANG Duo;TAN Kang;YAN Chun-wei;PENG Jun;PENG Qing-hua(Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine,Changsha 410208,China;Liuyang Jili Hospital,Liuyang 410300,China;Ophthalmology Department,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心,长沙410208 [3]浏阳市集里医院,湖南浏阳410300 [4]湖南中医药大学第一附属医院眼科,长沙410007

出　　处：《中华中医药杂志》2022年第5期2712-2717,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.30772824,No.81574031);中医药防治五官科疾病湖南省重点实验室建设项目(No.2017TP1018);国家中医药管理局中医眼科学重点学科建设项目(No.ZK1801YK015);湖南省研究生科研创新项目(No.CX2018B497,No.CX20200783);湖南中医药大学中医学国内一流建设学科建设项目,中医药防治眼耳鼻咽喉疾病湖南省重点实验室。

摘　　要：目的:研究肝经郁热型干眼患者与健康志愿者泪液之间的蛋白质组学差异。方法:纳入肝经郁热型干眼患者与健康志愿者各5例,采用数据非依赖性采集高分辨率色谱-质谱技术(DIA LC-MS)对肝经郁热型干眼患者与健康人泪液进行检测,筛选出差异蛋白,并对差异蛋白进行GO富集、KEGG通路、蛋白互作用网络等生物信息学分析。结果:干眼组与健康组比较共127个差异蛋白,其中115个上调,12个下调。肝经郁热型干眼患者差异蛋白主要涉及补体激活、经典途径,吞噬作用中的Fcγ受体信号通路等生物过程;涉及细胞外泌体、细胞外区域等细胞组分;涉及抗原结合、免疫球蛋白受体结合等分子功能。差异蛋白主要涉及PI3K-Akt信号通路、Fcγ受体介导的吞噬作用、原发性免疫缺陷等。肝经郁热组的重要节点蛋白有表皮生长因子受体、热休克70 kDa蛋白4、钙网蛋白等。结论:肝经郁热型干眼患者与健康志愿者泪液之间存在生物学差异,ENPP1有望成为肝经郁热型干眼的泪液生物标志物。Objective:To study the proteomic differences between tears of dry eye patients with liver meridian stagnation heat and healthy volunteers.Methods:Five dry eye patients with liver meridian stagnation heat and five healthy volunteers were enrolled.The tears were detected by data independent acquisition liquid chromatography-mass spectrometry(DIA LC-MS),and the differential proteins were screened out.The bioinformatics analysis of GO enrichment,KEGG pathway and protein interaction network were carried out.Results:Compared with the healthy group,there were 127 differential proteins in liver meridian stagnation heat group,of which 115 were up-regulated and 12 were down-regulated.Differential proteins in dry eyes patients with liver meridian stagnation heat mainly involved in biological processes such as complement activation,classical pathway,Fcγreceptor signaling pathway;Involved in cellular components such as extracellular exosome,extracellular region;Involved in molecular functions such as antigen binding,immunoglobulin receptor binding.The main differences of proteins involved in PI3K-Akt signaling pathway,Fcγr-mediated phagocytosis,primary immunodeficiency and so on.Important node proteins in liver meridian stagnation heat group include epidermal growth factor receptor,heat shock 70 kDa protein 4,calreticulin and so on.Conclusion:There are biological differences between dry eye patients with liver meridian stagnation heat and healthy people.ENPP1 is expected to become a tear biomarker for dry eye with liver meridian stagnation heat.

关 键 词：肝经郁热 干眼 泪液 蛋白质组学 生物标志物 ENPP1 

分 类 号：R276.7[医药卫生—中医五官科学]